Cytoskeleton structure and composition in choanoflagellates
Cytoskeleton structure and composition in choanoflagellates
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DOI：
10.1111/j.1550-7408.1998.tb04550.x
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发表时间：
1998-05-01
影响因子：
2.2
通讯作者：
Leadbeater, BSC
中科院分区：
文献类型：
--
作者：
Karpov, SA;Leadbeater, BSC
利用光学显微镜和电子显微镜相结合的方法，研究了卵形单囊藻（原生动物：Choanoflagellida肯特目，1880）细胞骨架的结构和组成。包括念珠状Desmarella的补充观察结果。单个前鞭毛的基体被第二个无鞭毛基体近端和直角包着。两个基体的边缘通过纤维桥连接。一个长的，狭窄的，条纹状的，纤维状的小根从无鞭毛的基体的下边缘向后延伸到高尔基体。它的大部分长度与扁平囊的表面相连。根微管从电子致密物质环放射状地穿过，电子致密物质环环绕鞭毛基体的远端。这些微管向外延伸约为细胞长度的三分之一。在每个领腱内是由肌动蛋白组成的纵向微丝束，如荧光显微镜下罗丹明-鬼笔环肽染色所示。每个微丝束的基部通过细纤维桥与一个或多个小根微管相连。微管和腱微丝之间的附着进一步证明了当细胞骨架脱落时它们的协调位移。细胞骨架的作用，在维持的立场，衣领触角在间期和细胞分裂进行了讨论。
The structure and composition of the cytoskeleton has been studied in Monosiga ovata (Protozoa: Order Choanoflagellida Kent 1880) using a combination of methods in association with light and electron microscopy. Supplementary observations are included for Desmarella moniliformis. The basal body of the single anterior flagellum is subtended proximally and at right angles by a second, nonflagellar basal body. The edges of the two basal bodies are connected by a fibrillar bridge. A long, narrow, striated, fibrillar rootlet extends posteriorly from the lower edge of the non-flagellar basal body towards the Golgi apparatus. It is associated throughout most of its length with the surface of a flattened sac. Rootlet microtubules pass radially from a ring of electron dense material which encircles the distal end of the flagellar basal body. These microtubules extend outwards for about one-third of the length of the cell. Within each collar tentacle is a longitudinal bundle of microfilaments composed of actin as illustrated by rhodamine-phalloidin staining for fluorescence microscopy. The base of each microfilament bundle is associated with one or more rootlet microtubules by fine fibrillar bridges. The attachment between microtubules and tentacle microfilaments is further demonstrated by their coordinated displacement when the cytoskeleton becomes dislodged. The role of the cytoskeleton in maintaining the position of the collar tentacles during interphase and cell division is discussed.