Rhodamine B accumulation and MXR protein expression in mussel blood cells: Effects of exposure to vincristine
Rhodamine B accumulation and MXR protein expression in mussel blood cells: Effects of exposure to vincristine
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DOI：
10.3354/meps142165
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发表时间：
1996-10-01
影响因子：
2.5
通讯作者：
Moore, MN
中科院分区：
文献类型：
--
作者：
Minier, C;Moore, MN
贻贝血细胞在其溶酶体室中积累罗丹明B。通过计算机共聚焦显微镜检测和定量的这种细胞内滞留通过维拉帕米的存在而增强。2个不同人群的研究表明，罗丹明B保留和同时差异表达水平的蛋白质免疫相关的多药耐药（MDR）转运蛋白在其环境中的变化。动物长期暴露于抗肿瘤生物碱长春新碱导致血细胞积累较少的罗丹明B，对维拉帕米更敏感，并表达更多的MDR相关蛋白。这些数据增加了一致性，以前的结果在贻贝的多异生素抗性。
Mussel hemocytes accumulate rhodamine B within their lysosomal compartment. This intracellular retention, detected and quantified by computerised confocal microscopy, is enhanced by the presence of verapamil. Study of 2 different populations showed alteration in rhodamine B retention and simultaneous differential expression levels of proteins immunorelated to the multidrug resistance (MDR) transporter in relation to their environment. Chronic exposure of animals to the Vinca antitumor alkaloid vincristine causes the hemocytes to accumulate less rhodamine B, to be more sensitive to verapamil and to express more MDR-related proteins. These data add consistency to previous results on multixenobiotic resistance in mussels.