MOLECULAR-CLONING AND CHARACTERIZATION OF A FULL-LENGTH CDNA CLONE FOR HUMAN-PLASMINOGEN
MOLECULAR-CLONING AND CHARACTERIZATION OF A FULL-LENGTH CDNA CLONE FOR HUMAN-PLASMINOGEN
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DOI：
10.1016/0014-5793(87)81501-6
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发表时间：
1987-03-23
期刊：
影响因子：
3.5
通讯作者：
HEDEN, LO
中科院分区：
文献类型：
--
作者：
FORSGREN, M;RADEN, B;HEDEN, LO
利用合成的24个核苷酸的探针，从已报道的部分c DNA序列中筛选出一个富含全长克隆的人肝脏c DNA文库，以寻找纤溶酶原c DNA。共鉴定出12个阳性克隆，并对其中一个克隆进行了详细鉴定。2.7kb的插入片段包含完整的编码区。在5个位置，它给出了与先前蛋白质氨基酸序列分析中报道的不同的残基。目前的结果显示，在65位有额外的LLE，在53和342位用Gln代替Glu，在88位用Asn代替Asp，在453位用Asp代替Asn。在3‘-非编码区，发现了29个碱基的延伸，它不包含任何与已知的多聚腺苷化信号相容的结构。相反，共识信号AATAAA被放置在聚(A)-尾上游46个碱基的距离处。
A human liver cDNA library enriched for full‐length clones was screened for plasminogen cDNA using a synthetic 24‐nucleotide probe derived from a reported partial cDNA sequence. 12 positive clones were identified and one of these was characterized in detail. The 2.7 kb insert contains the complete coding region. At 5 positions, it gives residues different from those reported in a previous amino acid sequence analysis of the protein. The present results show an extra lle at position 65, Gln instead of Glu at positions 53 and 342, Asn at position 88 instead of Asp, and Asp at position 453 rather than Asn. In the 3'‐non‐coding region an extension of 29 bases is found which does not contain any structure compatible with a known polyadenylation signal. Instead, the consensus signal AATAAA is placed at a distance of 46 bases upstream of the poly(A)‐tail.