Cellular Fragments as Biomaterial for Rapid In Vitro Bone-Like Tissue Synthesis
Cellular Fragments as Biomaterial for Rapid In Vitro Bone-Like Tissue Synthesis
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DOI：
10.3390/ijms21155327
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发表时间：
2020-08-01
影响因子：
5.6
通讯作者：
Matsumoto, Takuya
中科院分区：
文献类型：
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作者：
Akhter, Mst Nahid;Hara, Emilio Satoshi;Matsumoto, Takuya
目前基于干细胞的类骨组织合成技术至少需要两到三周的时间。因此，仍然需要新的技术来促进体外快速的三维类骨组织合成。在这项研究中，我们探索了使用细胞纳米碎片作为基质材料来促进体外快速成骨的概念。制备细胞纳米膜的方法有超声(30 S，3min)、非离子洗涤剂(Triton 0.1%和1%)或冻干粉。结果表明，超声作用3min可以得到长度小于150 nm的均匀纳米碎片，其矿化速度比其他方法快得多，仅需一天时间。对培养条件的进一步优化表明，10 mM或100 mM的β-甘油磷酸可以促进细胞纳米膜的矿化，而胎牛血清(FBS)则以浓度依赖的方式抑制细胞纳米膜的矿化。最后，通过将细胞纳米碎片结合在胶原凝胶中，在短短两天内就生成了模拟骨样结构的3D胶原-细胞纳米碎片-矿物复合体。综上所述，超声作用3min是一种制备长度小于150 nm的细胞纳米片的新方法，可作为体外骨组织工程的材料。
Current stem cell-based techniques for bone-like tissue synthesis require at least two to three weeks. Therefore, novel techniques to promote rapid 3D bone-like tissue synthesis in vitro are still required. In this study, we explored the concept of using cell nanofragments as a substrate material to promote rapid bone formation in vitro. The methods for cell nanofragment fabrication were ultrasonication (30 s and 3 min), non-ionic detergent (triton 0.1% and 1%), or freeze-dried powder. The results showed that ultrasonication for 3 min allowed the fabrication of homogeneous nanofragments of less than 150 nm in length, which mineralized surprisingly in just one day, faster than the fragments obtained from all other methods. Further optimization of culture conditions indicated that a concentration of 10 mM or 100 mM of beta-glycerophosphate enhanced, whereas fetal bovine serum (FBS) inhibited in a concentration-dependent manner, the mineralization of the cell nanofragments. Finally, a 3D collagen-cell nanofragment-mineral complex mimicking a bone-like structure was generated in just two days by combining the cell nanofragments in collagen gel. In conclusion, sonication for three min could be applied as a novel method to fabricate cell nanofragments of less than 150 nm in length, which can be used as a material for in vitro bone tissue engineering.