Chondrogenic potential of mesenchymal stromal cells derived from equine bone marrow and umbilical cord blood
Chondrogenic potential of mesenchymal stromal cells derived from equine bone marrow and umbilical cord blood
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DOI：
10.3415/vcot-08-10-0107
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发表时间：
2009-01-01
影响因子：
1.3
通讯作者：
Betts, D. H.
中科院分区：
文献类型：
--
作者：
Berg, L. C.;Koch, T. G.;Betts, D. H.
目的：骨科损伤是导致马术运动员训练天数减少或提前退役的最常见原因。细胞疗法是一种潜在的治疗肌肉骨骼疾病的新选择。间充质间质细胞（MSC）来源于马的多种来源，包括骨髓和脐带血。本研究的目的是在体外比较来自成人骨髓（BM-MSC）和脐带血（CB-MSC）的间充质间质细胞（MSC）的成软骨潜能。结果：两种来源的间充质干细胞产生的组织具有软骨样形态，蛋白聚糖染色阳性，并表达软骨标志物。早在第6天，CB-MSC颗粒较大，呈现透明样软骨形态。21型胶原蛋白、聚集蛋白和CD-RAP基因在CB- msc颗粒中的表达高于BM-MSC颗粒。Sox9 mRNA在CB-和BM-MSC颗粒中的表达相似。CB-培养基中软骨源性维甲酸敏感蛋白的蛋白浓度高于BM-MSC微球。结论：CB-MSC和BM-MSC均能在体外形成透明样软骨。然而，在这项研究中，基于测试的细胞和测量的参数，来自脐带血的MSC似乎比BM-MSC具有更大的成软骨潜力。
Objective: Orthopaedic injury is the most common cause of lost training days or premature retirement in the equine athlete. Cell-based therapies are a potential new treatment option in musculo-skeletal diseases. Mesenchymal stromal cells (MSC) have been derived from multiple sources in the horse including bone marrow and umbilical cord blood, The objective of this study was to provide an in vitro comparison of the chondrogenic potential in MSC derived from adult bone marrow (BM-MSC) and umbilical cord blood (CB-MSC).Results: MSC from both sources produced tissue with cartilage-like morphology that stained positive for proteoglycans and expressed cartilage markers. The CB-MSC pellets were larger and showed hyaline-like cartilage morphology as early as day six. Gene expression of collagen type 21, aggrecan and CD-RAP was higher in CB- than BM-MSC pellets. Expression of Sox9 mRNA was similar between CB- and BM-MSC pellets. Protein concentration of cartilage-derived retinoic acid sensitive protein was higher in culture medium from CB- than BM-MSC pellets.Conclusion: CB-MSC and BM-MSC were both capable of producing hyaline-like cartilage in vitro. However, in this study the MSC from umbilical cord blood appeared to have more chondrogenic potential than the BM-MSC based on the cells tested and parameters measured.