Evidence of Resistance to Cry34/35Ab1 Corn by Western Corn Rootworm (Coleoptera: Chrysomelidae): Root Injury in the Field and Larval Survival in Plant-Based Bioassays
Evidence of Resistance to Cry34/35Ab1 Corn by Western Corn Rootworm (Coleoptera: Chrysomelidae): Root Injury in the Field and Larval Survival in Plant-Based Bioassays
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DOI：
10.1093/jee/tow110
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发表时间：
2016-08-01
影响因子：
2.2
通讯作者：
St Clair, Coy R.
中科院分区：
文献类型：
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作者：
Gassmann, Aaron J.;Shrestha, Ram B.;St Clair, Coy R.
西部玉米根虫Diabrotica virgifera virgifera LeConte(鞘翅目：叶甲科)是美国玉米上的一种重要害虫，近年来对西部玉米根虫的治理包括种植Bt玉米。从2009年开始，在爱荷华州和其他地方发现了对Cry3Bb1玉米和mCry3A玉米具有抗性的西部玉米根虫种群。到目前为止，西部玉米根虫种群仍然对产生Bt毒素Cry34/35Ab1的玉米敏感。在这项研究中，我们使用单植物生物测定方法测试了西部玉米根虫对Cry34/35Ab1玉米、Cry3Bb1玉米和mCry3A玉米的抗性。生物测定包括从观察到Bt玉米受到严重伤害的田地收集的9个根虫种群，以及从未接触过Bt玉米的6个对照种群。我们在田间群体中发现了对Cry34/35Ab1玉米的不完全抗性，这些群体来自那些对产生Cry34/35Ab1的玉米产生严重伤害的田间群体，无论是单株还是金字塔状。此外，在大多数供试种群中发现了对Cry3Bb1玉米和mCry3A玉米的抗性。这些对Cry34/35Ab1玉米的抗性，以及西部玉米根虫对多种Bt毒素的抗性的存在，突显了Bt玉米对西部玉米根虫抗性进化的潜在脆弱性。除了抗虫性管理外，使用更多样化的管理方法，可能是维持Bt玉米在管理西部玉米根虫方面的生存能力所必需的。
Western corn rootworm, Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae), is a serious pest of corn in the United States, and recent management of western corn rootworm has included planting of Bt corn. Beginning in 2009, western corn rootworm populations with resistance to Cry3Bb1 corn and mCry3A corn were found in Iowa and elsewhere. To date, western corn rootworm populations have remained susceptible to corn producing Bt toxin Cry34/35Ab1. In this study, we used single-plant bioassays to test field populations of western corn rootworm for resistance to Cry34/35Ab1 corn, Cry3Bb1 corn, and mCry3A corn. Bioassays included nine rootworm populations collected from fields where severe injury to Bt corn had been observed and six control populations that had never been exposed to Bt corn. We found incomplete resistance to Cry34/35Ab1 corn among field populations collected from fields where severe injury to corn producing Cry34/35Ab1, either singly or as a pyramid, had been observed. Additionally, resistance to Cry3Bb1 corn and mCry3A corn was found among the majority of populations tested. These first cases of resistance to Cry34/35Ab1 corn, and the presence of resistance to multiple Bt toxins by western corn rootworm, highlight the potential vulnerability of Bt corn to the evolution of resistance by western corn rootworm. The use of more diversified management practices, in addition to insect resistance management, likely will be essential to sustain the viability of Bt corn for management of western corn rootworm.