The adult abdominal neuromuscular junction of Drosophila:: A model for synaptic plasticity
The adult abdominal neuromuscular junction of Drosophila:: A model for synaptic plasticity
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DOI：
10.1002/neu.20279
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发表时间：
2006-09-01
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影响因子：
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通讯作者：
Fernandes, Joyce J.
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文献类型：
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作者：
Hebbar, Sarita;Hall, Rachel E.;Fernandes, Joyce J.
果蝇在其生命周期中形成两组神经肌肉接头（NMJ），即胚胎/幼虫和成虫，它们具有不同的阶段特异性功能。在变态过程中，幼虫NMJ被重组以产生它们的成年对应物，这一过程被整合到神经系统的整体重塑中。前面已经描述了前胸肌和中胸背纵（飞行）肌的NMJ。鉴于成人肌肉群的多样性和复杂性，我们开始检查不太复杂的腹肌。这些NMJ的大的终扣尺寸对于容易可视化是特别有利的。具体来说，我们的特征腹侧腹NMJ的形态属性，并表明，胚胎运动神经元的身份基因，dHb 9，在这些成人交界处表达。我们量化了终扣的数量和大小，并检查了突触标记的定位。我们还检查了变态过程中纽扣的形成，并检查了这些阶段突触前标记的定位。为了测试腹侧NMJ作为模型系统的有用性，我们表征了改变电活动和细胞粘附分子Fascicli-nII（FasH）水平的影响。我们表明，这两种操作影响NMJ的形成和效果是具体的，因为它们可以被拯救的遗传。我们的研究结果表明，活动和FasH影响成人腹部NMJ的发育，其方式与幼虫和成人胸部对应物不同（c）2006 Wiley Periodicals，Inc.
During its life cycle, Drosophila makes two sets of neuromuscular junctions (NMJs), embryonic/ larval and adult, which serve distinct stage-specific functions. During metamorphosis, the larval NMJs are restructured to give rise to their adult counterparts, a process that is integrated into the overall remodeling of the nervous system. The NMJs of the prothoracic muscles and the mesothoracic dorsal longitudinal (flight) muscles have been previously described. Given the diversity and complexity of adult muscle groups, we set out to examine the less complex abdominal muscles. The large bouton sizes of these NMJs are particularly advantageous for easy visualization. Specifically, we have characterized morphological attributes of the ventral abdominal NMJ and show that an embryonic motor neuron identity gene, dHb9, is expressed at these adult junctions. We quantified bouton numbers and size and examined the localization of synaptic markers. We have also examined the formation of boutons during metamorphosis and examined the localization of presynaptic markers at these stages. To test the usefulness of the ventral abdominal NMJs as a model system, we characterized the effects of altering electrical activity and the levels of the cell adhesion molecule, Fascicli-nII (FasH). We show that both manipulations affect NMJ formation and that the effects are specific as they can be rescued genetically. Our results indicate that both activity and FasH affect development at the adult abdominal NMJ in ways that are distinct from their larval and adult thoracic counterparts (c) 2006 Wiley Periodicals, Inc.