Enzymatic synthesis of deoxyribonucleic acid. XXIX. Hydrolysis of deoxyribonucleic acid from the 5' terminus by an exonuclease function of deoxyribonucleic acid polymerase.
Enzymatic synthesis of deoxyribonucleic acid. XXIX. Hydrolysis of deoxyribonucleic acid from the 5' terminus by an exonuclease function of deoxyribonucleic acid polymerase.
复制标题
脱氧核糖核酸的酶促合成。
DOI：
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发表时间：
1969
影响因子：
4.8
通讯作者：
A. Kornberg
中科院分区：
文献类型：
--
作者：
M. Deutscher;A. Kornberg
Abstract The hydrolytic activity associated with homogeneous preparations of Escherichia coli DNA polymerase, previously designated exonuclease II, hydrolyzes polydeoxyribonucleotides from the 5' as well as the 3' terminus. 3'-Phosphate-terminated DNA is hydrolyzed only from the 5' terminus. The principal products of exhaustive hydrolysis of 3'-phosphate-terminated DNA are 5'-mononucleotides and oligonucleotides terminating in a 3'-phosphate group; the latter are not hydrolyzed from either end of the chain. The rates of hydrolysis from either end of a DNA chain are similar with native DNA as substrate. The ability of DNA polymerase to recognize and degrade from the 5' terminus (5' → 3') as well as the 3' terminus (3' → 5') of a DNA chain is considered in relation to possible models for DNA replication and to the active center of the enzyme.
