Fluorophore labeling of core-crosslinked polymeric micelles for multimodal in vivo and ex vivo optical imaging.
Fluorophore labeling of core-crosslinked polymeric micelles for multimodal in vivo and ex vivo optical imaging.
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DOI：
10.2217/nnm.14.170
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Lammers T
中科院分区：
文献类型：
--
作者：
Shi Y;Kunjachan S;Wu Z;Gremse F;Moeckel D;van Zandvoort M;Kiessling F;Storm G;van Nostrum CF;Hennink WE;Lammers T
使多模式体内和体外光学成像的生物分布和肿瘤积累的核心-交联型聚合物胶束(CCPM)。合成了以聚乙二醇单甲氧基硅氧烷(mpeg-b-p)(HPMAm-Lac)为基团，以半胺为核的共价标记的聚合物胶束(Dy-676/488)。给CT26荷瘤小鼠静脉注射CCPM。静脉注射CCPM后，CCPM在CT26肿瘤体内有效蓄积，24小时达~4%ID。体外TPLSM证实，iCCPM有效地渗出肿瘤血管，并深入肿瘤间质。CCPM被标记了多个荧光团，它们举例说明，结合不同的体内和体外光学成像技术对于分析纳米药物的生物分布和肿瘤蓄积非常有用。
To enable multimodal in vivo and ex vivo optical imaging of the biodistribution and tumor accumulation of core-crosslinked polymeric micelles (CCPM). mPEG-b-p(HPMAm-Lac)-based polymeric micelles, core-crosslinked via cystamine and covalently labeled with two fluorophores (Dy-676/488) were synthesized. The CCPM were intravenously injected in CT26 tumor-bearing mice. Upon intravenous injection, the CCPM accumulated in CT26 tumors reasonably efficiently, with values reaching ~4 %ID at 24 hours. Ex vivo TPLSM confirmed efficient extravasation of the iCCPM out of tumor blood vessels and deep penetration into the tumor interstitium. CCPM were labeled with multiple fluorophores, and they exemplify that combining different in vivo and ex vivo optical imaging techniques is highly useful for analyzing the biodistribution and tumor accumulation of nanomedicines.