Comparative Proteomic Analyses of Synovial Fluids and Serums from Rheumatoid Arthritis Patients
Comparative Proteomic Analyses of Synovial Fluids and Serums from Rheumatoid Arthritis Patients
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DOI：
10.4014/jmb.1307.07046
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发表时间：
2014-01-01
影响因子：
2.8
通讯作者：
Park, Byoung Chul
中科院分区：
文献类型：
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作者：
Noh, Ran;Park, Sung Goo;Park, Byoung Chul
风湿性关节炎（RA）是一种慢性全身性炎症性疾病，主要影响灵活的关节，也可能影响许多组织和器官。RA的进展涉及关节周围关节囊的炎症反应、具有过量滑液（SF）的滑膜细胞肿胀以及滑膜中纤维组织的发展。由于疾病的进行性病理学常导致关节软骨的不可逆破坏和关节强直，因此早期诊断RA至关重要。因此，我们进行了比较蛋白质组学的方法来研究新的生物标志物的早期诊断使用SF和血清RA患者。结果，我们鉴定出32个在SF中差异表达的斑点和34个在血清中差异表达的斑点。STEAP 4和ZNF 658蛋白的差异表达分别使用SF和血清的免疫印迹进行验证。这些数据表明，差异表达的蛋白质在SF和血清中可作为RA特异性生物标志物的诊断和监测RA。此外，这些发现推进了我们对RA的分子发病机制的理解。
Rheumatoid arthritis (RA) is a chronic and systemic inflammatory disorder that primarily affects the flexible joints and may also affect a number of tissues and organs. The progression of RA involves an inflammatory response of the capsule around the joint, swelling of synovial cells with excess synovial fluid (SF), and the development of fibrous tissue in the synovium. Since the progressive pathology of the disease often leads to the irreversible destruction of articular cartilage and ankylosis of the joint, early diagnosis of RA is essential. Thus, we undertook a comparative proteomic approach to investigate novel biomarkers for early diagnosis using SFs and serums from RA patients. As a result, we identified 32 differentially expressed spots in SFs and 34 spots in serums. The differential expression of the STEAP4 and ZNF 658 proteins were validated using immunoblotting of the SFs and serums, respectively. These data suggest that differentially expressed proteins in SFs and serums could be used as RA-specific biomarkers for the diagnosis and monitoring of RA. Furthermore, these findings advance our understanding of the molecular etiopathogenesis of RA.