Fluorogenic probes for imaging cellular phosphatase activity.
Fluorogenic probes for imaging cellular phosphatase activity.
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DOI：
10.1016/j.cbpa.2020.04.004
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发表时间：
2020-05
影响因子：
7.8
通讯作者：
Brandon S McCullough;A. Barrios
中科院分区：
文献类型：
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作者：
Brandon S McCullough;A. Barrios
在细胞、组织或生物体中可视化酶活性的能力可以大大提高我们对酶的生物学作用的理解。虽然细胞信号传导的许多方面是由可逆的蛋白质磷酸化控制的，但我们对所涉及的蛋白质磷酸酶的生物学作用的理解是有限的。在这里，我们概述了荧光探针的发展进展，荧光探针可用于可视化蛋白磷酸酶的活性。重大进展包括具有可见和近红外（近红外）激发和发射剖面的探针的发展，它提供了更大的组织和全动物成像能力。此外，基于肽的探针的发展为感兴趣的磷酸酶提供了一定的选择性。关键的挑战包括对磷酸酶家族的单个成员实现足够的选择性的困难，以及在广泛采用之前充分验证最佳探针的必要性。
The ability to visualize enzyme activity in a cell, tissue, or living organism can greatly enhance our understanding of the biological roles of that enzyme. While many aspects of cellular signaling are controlled by reversible protein phosphorylation, our understanding of the biological roles of the protein phosphatases involved is limited. Here, we provide an overview of progress toward the development of fluorescent probes that can be used to visualize the activity of protein phosphatases. Significant advances include the development of probes with visible and near-infrared (near-IR) excitation and emission profiles, which provides greater tissue and whole-animal imaging capabilities. In addition, the development of peptide-based probes has provided some selectivity for a phosphatase of interest. Key challenges involve the difficulty of achieving sufficient selectivity for an individual member of a phosphatase enzyme family and the necessity of fully validating the best probes before they can be adopted widely.