Differential induction of two potato genes, Stprx2 and StNAC, in response to infection by Phytophthora infestans and to wounding
Differential induction of two potato genes, Stprx2 and StNAC, in response to infection by Phytophthora infestans and to wounding
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DOI：
10.1023/a:1010639225091
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发表时间：
2001-01-01
影响因子：
5.1
通讯作者：
Boller, T
中科院分区：
文献类型：
--
作者：
Collinge, M;Boller, T
为了在分子水平上了解马铃薯和致病疫霉之间的相互作用，我们利用mRNA差异显示技术筛选了感染后早期诱导的基因。在筛选的20个cDNA克隆中，发现两个代表其转录水平在感染完整植物期间增加的植物基因。这两个基因在对创伤的反应上有着显著的不同。Stprx2是一种过氧化物酶，对创伤反应缓慢且短暂，其表达模式类似于gst 1，一个已被广泛描述的马铃薯病原诱导基因。第二个基因，StNAC，诱导迅速和强烈的创伤后，但不是全身。转录水平在约1小时后达到最大值，并在约1小时后恢复到基础水平。24小时StNAC与NAC结构域蛋白的ATAF亚家族（推定的转录激活因子的大家族）具有很强的相似性。拟南芥ATAF 1和ATAF 2也被证明是由创伤诱导。这意味着ATAF基因不仅在结构上相似，而且在应激反应中具有保守的作用。
To find out more about the interaction between potato and Phytophthora infestans at the molecular level, we screened for genes induced early after infection using mRNA differential display. Among the twenty cDNA clones recovered in the screen, two were found to represent plant genes whose transcript levels increased during infection of intact plants. These two genes differed strikingly in their response to wounding. Stprx2, a putative peroxidase, responded slowly and transiently to wounding, and its expression pattern was similar to that of gst1, a well-described pathogen-induced gene of potato. The second gene, StNAC, was induced rapidly and strongly after wounding but not systemically. Transcript levels reached a maximum after around 1 h and returned to basal levels after ca. 24 h. StNAC has strong similarity to the ATAF subfamily of NAC domain proteins, a large family of putative transcriptional activators. Arabidopsis ATAF1 and ATAF2 were also shown to be induced by wounding. This implies that the ATAF genes are not merely structurally similar but also share a conserved role in stress responses.