Effect of NeuroD2 expression on neuronal differentiation in mouse embryonic stem cells
Effect of NeuroD2 expression on neuronal differentiation in mouse embryonic stem cells
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DOI：
10.1016/j.cellbi.2008.10.010
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发表时间：
2009-02-01
影响因子：
3.9
通讯作者：
Nakanishi, Mamoru
中科院分区：
文献类型：
--
作者：
Sugimoto, Yasutaka;Furuno, Tadahide;Nakanishi, Mamoru
NeuroD2 是一种基本的螺旋-环-螺旋转录因子，在神经元分化和存活中发挥重要作用。我们将四环素依赖性 NeuroD2 表达系统引入胚胎干 (ES) 细胞，并研究 NeuroD2 在神经元分化中的作用。添加多西环素在ES细胞中诱导24小时后表达NeuroD2，并在3天后诱导向神经元的分化，ES细胞转染由具有巨细胞病毒启动子的反向四环素控制的反式激活子和具有四环素反应元件的NeuroD2组成的载体。用多西环素治疗 3 天可诱导神经元分化，但 1 天内不会诱导神经元分化；此外，治疗后3天在细胞核中检测到NeuroD2，但也不是在1天内检测到。结果表明，NeuroD2的表达需要大约3天的适当时间才能引发ES细胞中的神经元分化。 (C) 2008 年国际细胞生物学联合会。由爱思唯尔有限公司出版。保留所有权利。
A basic helix-loop-helix transcriptional factor, NeuroD2, plays important roles in neuronal differentiation and survival. We introduced the tetracycline-dependent NeuroD2 expression system to embryonic stem (ES) cells and studied the role of NeuroD2 in the neuronal differentiation. The addition of doxycycline induced the expression of NeuroD2 after 24 h and the differentiation to neurons after 3 days in ES cells, which are transfected with vectors composed of reverse tetracycline-controlled transactivator with cytomegarovirus promoter and NeuroD2 with tetracycline response element. Treatment with doxycycline for 3 days induced neuronal differentiation, but not within 1 day; furthermore NeuroD2 was detected in the nucleus 3 days after treatment, but also not within 1 day. The results suggest that the expression of NeuroD2 requires an appropriate period of about 3 days to elicit neuronal differentiation in ES cells. (C) 2008 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.