Quantitative developmental biology in vitro using micropatterning.

Quantitative developmental biology in vitro using micropatterning.
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DOI:
10.1242/dev.186387
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发表时间:
2021-08-01
期刊:
Development (Cambridge, England)
影响因子:
--
通讯作者:
Blin G
Blin G
中科院分区:
其他
文献类型:
--
作者:
Blin G

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微图案包含了一套旨在精确控制分子在材料表面的空间分布的方法。生物学家借鉴了这一理念,并调整了这些最初为电子学开发的方法,对生物系统施加物理约束,目的是解决从分子到多细胞系统等生物尺度上的基本问题。在这里,我从一个发育生物学家的角度来探讨这个话题,特别关注微图案是如何以及为什么近年来在发育生物学社区中流行起来的。总的来说,本入门提供了一个关于如何使用微模式来研究发育过程的简明概述,并强调了微模式如何成为发育生物学家工具箱中有用的补充。摘要:本入门介绍了微模式作为研究开发的工具,并讨论了我们迄今为止所学到的这种技术。
Micropatterning encompasses a set of methods aimed at precisely controlling the spatial distribution of molecules onto the surface of materials. Biologists have borrowed the idea and adapted these methods, originally developed for electronics, to impose physical constraints on biological systems with the aim of addressing fundamental questions across biological scales from molecules to multicellular systems. Here, I approach this topic from a developmental biologist's perspective focusing specifically on how and why micropatterning has gained in popularity within the developmental biology community in recent years. Overall, this Primer provides a concise overview of how micropatterns are used to study developmental processes and emphasises how micropatterns are a useful addition to the developmental biologist’s toolbox. Summary: This Primer introduces micropatterning as a tool to study development and discusses what we have learnt so far with this technique.
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