Resonance Raman imaging of macular pigment distributions in the human retina
Resonance Raman imaging of macular pigment distributions in the human retina
复制标题
DOI：
10.1364/josaa.25.000947
复制
发表时间：
2008-04-01
影响因子：
1.9
通讯作者：
Gellermann, Werner
中科院分区：
文献类型：
--
作者：
Sharifzadeh, Mohsen;Zhao, Da-You;Gellermann, Werner
我们描述了活体人眼黄斑色素（MP）分布的共振拉曼成像（RRI）。MP由抗氧化剂类胡萝卜素化合物叶黄素和玉米黄质组成，通常以相对于周边视网膜的高浓度存在于健康人黄斑中，并且被认为保护该重要的中心区域免受年龄相关性黄斑变性。我们证明，RRI是能够量化和成像的空间强烈变化的MP分布在人类视网膜。使用MP分子在488 nm处的激光激发，以及在MP的最强拉曼峰位置和在离峰位置处从视网膜发射回来的光的顺序照相机检测，在每次曝光的几分之一秒内以低于50 μ m的分辨率获得MP的RRI图。RRI成像可以用未放大的瞳孔进行，并为人类受试者中的MP分布提供了高度分子特异性的诊断成像方法。(C)2008年美国光学学会。
We describe resonance Raman imaging (RRI) of macular pigment (MP) distributions in the living human eye. MP consists of the antioxidant carotenoid compounds lutein and zeaxanthin, is typically present in high concentrations in the healthy human macula relative to the peripheral retina, and is thought to protect this important central region from age-related macular degeneration. We demonstrate that RRI is capable of quantifying and imaging the spatially strongly varying MP distribution in the human retina. Using laser excitation of the MP molecules at 488 nm, and sequential camera detection of light emitted back from the retina at the MP's strongest Raman peak position and at an off-peak position, RRI maps of MP are obtained at a resolution below 50 mu m within a fraction of a second per exposure. RRI imaging can be carried out with undilated pupils and provides a highly molecule-specific diagnostic imaging approach for MP distributions in human subjects. (C) 2008 Optical Society of America.