Bone repair analysis in a novel biodegradable hydroxyapatite/collagen composite implanted in bone.
Bone repair analysis in a novel biodegradable hydroxyapatite/collagen composite implanted in bone.
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DOI：
10.1097/01.id.0000173628.00705.d0
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发表时间：
2005-09-01
影响因子：
--
通讯作者：
Tanaka, Akio
中科院分区：
文献类型：
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作者：
Nishikawa, Tetsunari;Masuno, Kazuya;Tanaka, Akio
本研究的目的是评估一种可生物降解的羟基磷灰石/胶原复合材料，并检查所含钙离子对骨形成和生长的作用。在Beagle犬股骨和胫骨上制备手术孔，用茜素红标记的羟基磷灰石/胶原复合材料填充。4周后，对实验犬给予钙黄绿素。再过1周后，手术取出股骨和胫骨，并固定在福尔马林中。采用光学显微镜和共聚焦激光扫描显微镜观察手术孔及其植入材料和周围骨组织。羟基磷灰石/胶原复合材料周围仅有少量炎性细胞。皮质骨中新形成的骨用钙黄绿素染色，其结合血清钙，孔中羟基磷灰石/胶原复合物附近的新骨对茜素红染色呈阳性，其结合羟基磷灰石/胶原复合物中的钙。此外，羟基磷灰石/胶原复合物附近的成骨细胞以及与成骨细胞相邻的新形成的骨显示茜素红染色，但距离羟基磷灰石/胶原植入物一定距离的新骨仅对钙黄绿素染色反应。这些结果，使用钙黄绿素和茜素红的组织标记方法，表明从羟基磷灰石/胶原复合材料释放的茜素红结合的钙可能已经移位到新骨形成的位点。本实验表明，新的羟基磷灰石/胶原复合物是一种有用的骨增量植入材料，新形成的骨中的钙可能已经从植入物中释放出来。
The purpose of this study was to evaluate a biodegradable hydroxyapatite/collagen composite and to examine the use of the calcium ion contained for bone formation and growth. Surgical holes were prepared in the femora and tibiae of beagle dogs, and were filled with the hydroxyapatite/collagen composite labeled with alizarin red. After 4 weeks, calcein was administered to the experimental dogs. After 1 additional week, the femora and tibiae were removed surgically and fixed in formalin. Light microscopy and confocal laser scanning microscopy were used to examine the surgical holes with their implanted materials and the surrounding bone. There were only a few inflammatory cells adjacent to the hydroxyapatite/collagen composite. The newly formed bone in the cortical bone was stained with calcein, which binds to serum calcium, and new bone near the hydroxyapatite/collagen composite in the holes was stained positive for alizarin red, which binds to the calcium in the hydroxyapatite/collagen composite. In addition, osteoblasts near the hydroxyapatite/collagen composite as well as newly formed bone adjacent to the osteoblasts showed alizarin red staining, but the new bone at a distance from the hydroxyapatite/collagen implant reacted only to calcein staining. These results, using the tissue labeling method with calcein and alizarin red, suggested that the calcium bound to the alizarin red released from the hydroxyapatite/collagen composite materials might have been translocated to sites of new bone formation. The present experiment showed that the novel hydroxyapatite/collagen composite is a useful implant material for bone augmentation and that the calcium in the newly formed bone might have been released from the implant.