BUFFER COMBINATIONS FOR MAMMALIAN CELL CULTURE
BUFFER COMBINATIONS FOR MAMMALIAN CELL CULTURE
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DOI：
10.1126/science.174.4008.500
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发表时间：
1971-01-01
期刊：
影响因子：
56.9
通讯作者：
EAGLE, H
中科院分区：
文献类型：
--
作者：
EAGLE, H
培养的哺乳动物细胞的生长和代谢明显受到普通碳酸氢盐缓冲培养基（pH 8.0至6.9）中pH变化的影响。通过两种或三种有机缓冲液（每种为10至15毫摩尔）与磷酸盐和碳酸氢盐的适当组合，可以减少这些pH波动，并且可以根据需要将培养物的pH稳定在pH 6.4至8.3的范围内。然后，稀疏培养物中的初始碱化被最小化，并且除了重培养物之外，24小时内的代谢酸化通常小于0.4pH单位。
The growth and metabolism of cultured mammalian cells are markedly affected by thepH variation in ordinary bicarbonate-buffered media (pH 8.0 to 6.9). ThosepH swings can be reduced and thepH of the culture can be stabilized as desired in the rangepH 6.4 to 8.3 by appropriate combinations of two or three organic buffers, each at 10 to 15 millimolar, in conjunction with phosphate and bicarbonate. The initial alkalinization in sparse cultures is then minimized, and the metabolic acidification in 24 hours is usually less than 0.4pH unit except in heavy cultures.