Construction of Active Protein Materials: Manipulation on Morphology of Salmon Calcitonin Assemblies with Enhanced Bone Regeneration Effect
Construction of Active Protein Materials: Manipulation on Morphology of Salmon Calcitonin Assemblies with Enhanced Bone Regeneration Effect
复制标题
活性蛋白材料的构建：通过调控鲑鱼降钙素组件的形态来增强骨再生效果
DOI：
10.1002/adma.202207526
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发表时间：
2022-09
影响因子：
29.4
通讯作者：
Guosong Chen
中科院分区：
文献类型：
--
作者：
Libin Wu;Liang Cheng;Jing Yang;Yufei Yan;Ensong Zhang;Zdravko Kochovski;Long Li;Zhen Wang;Lianfu Deng;Yan Lu;Pol Besenius;Wenguo Cui;Guosong Chen
蛋白质药物的作用总是受到其相对较低的稳定性和快速降解性质的限制;因此，已经做出各种优雅的努力来改善蛋白质药物的生物活性和生物相容性。在这里，提出了一种替代方法来解决这个问题。通过简单地添加有限量的小分子调节剂，调节蛋白质-蛋白质相互作用（PPI）和二硫键形成的微妙平衡，可以调节蛋白质药物的自组装特性，形成“活性蛋白质材料”本身。这意味着，所得生物材料以蛋白质药物和水为主，通过蛋白质与受体之间的多价效应以及组装蛋白质的延缓降解，与原始蛋白质相比，在体外和体内具有显着增强的骨再生效果。在这种活性蛋白材料中，蛋白质药物不仅是活性药物，而且是药物载体，大大提高了生物材料的载药效率，表明活性蛋白材料具有制备简单、效率高、成本低的优点，在生物医学应用中具有广阔的前景。
The effect of protein drugs is always limited by their relatively low stability and fast degradation property; thus, various elegant efforts have been made to improve the bioactivity and biocompatibility of the protein drugs. Here, an alternative way is proposed to solve this problem. By simply adding a limited amount of small‐molecular regulator, which tunes the subtle balance of protein–protein interactions (PPIs) and disulfide bond formation, the self‐assembly property of the protein drug can be regulated, forming an “active protein material” itself. This means that, the resulting biomaterial is dominated by the protein drug and water, with significantly enhanced bone regeneration effect compared to the virgin protein in vitro and in vivo, through multivalent effect between the protein and receptor and the retarded degradation of the assembled proteins. In this active protein material, the protein drug is not only the active drug, but also the drug carrier, which greatly increases the drug‐loading efficiency of the biomaterial, indicating the advantages of the easy preparation, high efficiency, and low cost of the active protein material with a bright future in biomedical applications.