Nitrogen-dependent luteolin effect on Microcystis growth and microcystin-pollution risk — Novel mechanism insights unveiled by comparative proteomics and gene expression
Nitrogen-dependent luteolin effect on Microcystis growth and microcystin-pollution risk — Novel mechanism insights unveiled by comparative proteomics and gene expression
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氮依赖性木犀草素对微囊藻生长和微囊藻毒素污染风险的影响 – 通过比较蛋白质组学和基因表达揭示的新机制见解
DOI：
10.1016/j.envpol.2022.119848
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发表时间：
2022
影响因子：
8.9
通讯作者：
Guo Zhonghui
中科院分区：
文献类型：
--
作者：
An Guangqi;Li Jieming;Lu Haifeng;Guo Zhonghui
植物源化感物质木樨草素具有抑制微囊藻水华（MCBs）作用，但其抑制微囊藻水华的作用受自然因素影响，尤其是受沃茨中氮（N）水平的影响。本研究从蛋白质组学和基因表达的角度探讨了N-依赖的木樨草素对微囊藻生长及其毒素（MCs）产生/释放的影响，并阐明了其作用机制。比较蛋白质组学和基因表达研究表明，在不同氮水平下，毛地黄黄酮剂量的增加通过抑制光捕获、光合电子传递、卡尔文循环和磷（P）获取等蛋白质的合成和基因表达，逐渐抑制微囊藻的生长。在较高的木樨草素浓度和较低的氮水平下，微囊藻细胞的微囊藻毒素（MCs）产生量和保存能力都有增加的趋势，在12 mg/L木樨草素和0.5mg/L氮水平下，第10天的增加幅度最大，分别达到对照的1.96和2.68倍，但随着胞外MCs含量的增加，MCs的释放量减少，在12 mg/L木樨草素胁迫下，N水平升高对水稻幼苗生长的抑制率分别为72.86%、73.57%、74.45%和40.58%、45.28%、60.00%。这使得微囊藻对更强的胁迫和氮限制做出防御反应。在木樨草素胁迫下，较高的N水平更强烈地上调了许多过程（例如，氧化还原酶活性、ATP结合和跨膜转运、氧化磷酸化、三羧酸循环、脂肪酸生物合成、糖酵解/糖异生、丙酮酸、氨基酸代谢、金属离子结合、磷的获得）作为对高氮水平下光合和核糖体过程进行性下调的补偿性保护反应，从而导致微囊藻比低氮水平下生长更快。本研究为毛地黄黄酮在不同富营养化程度沃茨中的杀藻应用提供了理论依据。
hytogenic allelochemical luteolin has potential to mitigate Microcystis-dominated cyanobacterial blooms (MCBs), but its algicidal effect against toxigenic Microcystis may be impacted by natural factors, especially ni-trogen (N) level in waters. This study innovatively explored N-dependent effect of luteolin on Microcystis growth and its microcystins (MCs) production/release, and elucidated underlying mechanisms from proteomics and gene expression views. Generally, at each N level, rising luteolin dose progressively inhibited Microcystis growth by inhibiting proteins syntheses and genes expression involving light-capturing, photosynthetic electron transfer, Calvin cycle and phosphorus (P) acquisition, according to comparative proteomics and gene expression. At higher luteolin dose and lower N level, Microcystis cell tended to increase microcystins (MCs) production and conservation ability, with the highest increase degree observed at 12 mg/L luteolin and 0.5 mg/L N on day 10, reaching 1.96 and 2.68 folds of luteolin-free control, respectively, but decrease MC-release as extracellular MCs content (EMC), with inhibition ratio of 72.86%, 73.57%, 74.45% and 40.58%, 45.28%, 60.00% at rising N level under 12 mg/L luteolin stress on day 10 and 16, respectively. These enabled cellular defensive response of Microcystis to stronger stress and N limitation. Under luteolin stress, higher N level more strongly up-regulated numerous processes (e.g., oxidoreductase activity, ATP binding and transmembrane transport, oxidative phos-phorylation, tricarboxylic acid cycle, fatty acid biosynthesis, glycolysis/gluconeogenesis, pyruvate, amino acids metabolism, metal ion-binding, P acquisition) as compensative protective responses to progressively down -regulated photosynthetic and ribosomal processes at higher N level, thus causing faster Microcystis growth than lower N level. This study provided novel insights for N-dependent effect and mechanisms of luteolin on MCBs mitigation and MCs risk control, and guided algicidal application of luteolin in different eutrophic-degree waters.