NEW NORFLOXACIN RESISTANCE GENE IN PSEUDOMONAS-AERUGINOSA PAO
NEW NORFLOXACIN RESISTANCE GENE IN PSEUDOMONAS-AERUGINOSA PAO
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DOI：
10.1128/aac.34.9.1757
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发表时间：
1990-09-01
影响因子：
4.9
通讯作者：
IYOBE, S
中科院分区：
文献类型：
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作者：
FUKUDA, H;HOSAKA, M;IYOBE, S
从铜绿假单胞菌中分离到一株新的去甲藻素突变株PAO。该突变体显示对亚胺培南和氯霉素的交叉抗性和对β-内酰胺类抗生素的超敏感性。内酰胺类和氨基糖苷类抗生素。将新的诺氟沙星耐药基因nfxC定位在PAO染色体上。诺氟沙星积累减少nfsC突变体，此外，亚胺培南扩散通过外膜的nfxC突变体的速率低于亲本菌株。钠46千道尔顿蛋白和增加的50千道尔顿蛋白的nfxC突变体。我们的结论是nfxC是一个新的诺氟沙星耐药基因，影响外膜通透性喹啉和其他抗菌药物。
A new type of norfloxacin-mutant of Pseudomonas aeruginosa PAO was isolated. This mutant showed cross resistance to imipenem and chloramphenicol and hypersusceptibility to .beta.-lactam and aminoglycoside antibiotics. The new norfloxacin resistance gene nfxC was mapped hear catA (46 min) on the PAO chromsoome. Norfloxacin accumulation was decreased in the nfsC mutant; furthermore, the rate of imipenem diffusion through the outer membrane of the nfxC mutant was lower than of the parent strain. Sodium 46-kilodalton protein and an increase of a 50-kilodalton protein in the nfxC mutant. We conclude the nfxC is a new norfloxacin resistance gene that affects outer membrane permeability to quinolines and other antimicrobial agents.