The use of osmium-thiocarbohydrazide for structural stabilization and enhancement of secondary electron images in scanning electron microscopy of pollen
The use of osmium-thiocarbohydrazide for structural stabilization and enhancement of secondary electron images in scanning electron microscopy of pollen
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DOI：
10.1080/00173139509429065
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发表时间：
1995-10-01
期刊：
影响因子：
0.9
通讯作者：
Skvarla, JJ
中科院分区：
文献类型：
--
作者：
Chissoe, WF;Vezey, EL;Skvarla, JJ
在扫描电子显微镜(SEM)中，染色在一系列Os(O)和硫代碳肼(T)溶液(统称为Ototo)中的花粉颗粒结构稳定且不扭曲，通常不需要特殊干燥。事实上，Ototo可以被视为孢粉学中的另一种特殊的干燥方法。这种连续的培养也显著提高了扫描电子显微镜下花粉粒的电导率。与标准的溅射或真空蒸发程序相比，Ototo减少了充电，并产生分辨率显著更高的二次电子图像。
Pollen grains stained in a sequence of osmium (O) and thiocarbohydrazide (T) solutions (collectively known as OTOTO) appear structurally stable and undistorted in the scanning electron microscope (SEM), and usually do not require special drying. In fact, OTOTO can be regarded as another special drying method in palynology. This sequential incubation also strikingly increases the electrical conductivity of pollen grains in the SEM. Compared to standard sputter-coating or vacuum evaporative procedures, OTOTO reduces charging and yields secondary electron images with significantly higher resolution.