Comparative synteny cloning of zebrafish you-too:: mutations in the Hedgehog target gli2 affect ventral forebrain patterning
Comparative synteny cloning of zebrafish you-too:: mutations in the Hedgehog target gli2 affect ventral forebrain patterning
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DOI：
10.1101/gad.13.4.388
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发表时间：
1999-02-15
影响因子：
10.5
通讯作者：
Schier, AF
中科院分区：
文献类型：
--
作者：
Karlstrom, RO;Talbot, WS;Schier, AF
斑马鱼you-too（yot）突变干扰胚胎发育过程中的Hedgehog（Hh）信号传导。使用比较同线性的方法，我们分离出yot作为与Hh靶gli 2同源的锌指转录因子。yot的两个等位基因含有无义突变，导致羧基末端截短的蛋白质。除了引起中线发育、肌肉分化和视网膜轴突引导的缺陷外，yot突变还破坏垂体前叶和腹侧前脑的分化。yot突变还引起腹侧间脑中的异位透镜形成。这些研究结果表明，截短的斑马鱼Gli 2蛋白干扰Hh信号必需的分化和轴突的指导在腹侧前脑。
Zebrafish you-too (yot) mutations interfere with Hedgehog (Hh) signaling during embryogenesis. Using a comparative synteny approach, we isolated yot as a zinc finger transcription factor homologous to the Hh target gli2. Two alleles of yot contain nonsense mutations resulting in carboxy-terminally truncated proteins. In addition to causing defects in midline development, muscle differentiation, and retinal axon guidance, yot mutations disrupt anterior pituitary and ventral forebrain differentiation. yot mutations also cause ectopic lens formation in the ventral diencephalon. These findings reveal that truncated zebrafish Gli2 proteins interfere with Hh signaling necessary for differentiation and axon guidance in the ventral forebrain.