MICROTUBULES IN MESOPHYLL-CELLS OF NONACCLIMATED AND COLD-ACCLIMATED SPINACH - VISUALIZATION AND RESPONSES TO FREEZING, LOW-TEMPERATURE, AND DEHYDRATION
MICROTUBULES IN MESOPHYLL-CELLS OF NONACCLIMATED AND COLD-ACCLIMATED SPINACH - VISUALIZATION AND RESPONSES TO FREEZING, LOW-TEMPERATURE, AND DEHYDRATION
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DOI：
10.1104/pp.97.1.175
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发表时间：
1991-09-01
期刊：
影响因子：
7.4
通讯作者：
CARTER, JV
中科院分区：
文献类型：
--
作者：
BARTOLO, ME;CARTER, JV
研究了菠菜（Spinacia oleracea L. cv Bloomsdale）叶肉细胞皮层微管对冷冻、解冻、过冷和脱水的反应。使用间接免疫荧光显微镜的改进程序观察微管。将未驯化和冷驯化菠菜的叶切片缓慢冷冻到不同温度，冷冻时固定，并对微管进行免疫标记。非驯化和冷驯化细胞在结冰后都表现出几乎完全的微管解聚。在23℃解冻1小时后，未驯化和冷驯化细胞中的微管都重新聚合。然而，随着时间的推移，非驯化细胞中的微管再次解聚。由于冷冻缓慢的叶组织细胞中的微管受到脱水和零下温度的影响，这些应力分别施加，并注意到它们对微管的影响。过冷诱导非驯化和冷驯化细胞的微管解聚，但程度小于冷冻。将叶子切片在室温下暴露于山梨糖醇（一种穿透细胞壁的渗透剂）或聚乙二醇10,000（一种非穿透性渗透剂）的溶液中会引起微管解聚。低温和脱水的影响在产生所观察到的冷冻过程中的微管反应中大致是相加的。在非驯化细胞和冷驯化细胞之间，微管稳定性只有很小的差异。
Responses of cortical microtubules in spinach (Spinacia oleracea L. cv Bloomsdale) mesophyll cells to freezing, thawing, supercooling, and dehydration were assessed. Microtubules were visualized using a modified procedure for indirect immunofluorescence microscopy. Leaf sections of nonacclimated and cold-acclimated spinach were slowly frozen to various temperatures, fixed while frozen, and microtubules immunolabelled. Both nonacclimated and cold-acclimated cells exhibited nearly complete microtubule depolymerization after ice formation. After 1 hour thawing at 23-degrees-C, microtubules in both nonacclimated and cold-acclimated cells repolymerized. With time, however, microtubules in nonacclimated cells again depolymerized. Since microtubules in cells of leaf tissue frozen slowly are subjected to dehydration as well as subzero temperatures, these stresses were applied separately and their effects on microtubules noted. Supercooling induced microtubule depolymerization in both non-acclimated and cold-acclimated cells, but to a smaller extent than did freezing. Exposing leaf sections to solutions of sorbitol (a cell wall-penetrating osmoticum) or polyethylene glycol 10,000 (a nonpenetrating osmoticum) at room temperature caused microtubule depolymerization. The effects of low temperature and dehydration are roughly additive in producing the observed microtubule responses during freezing. Only small differences in microtubule stability were resolved between nonacclimated and cold-acclimated cells.