Intraocular Expression of Serum Amyloid A and Interleukin-6 in Proliferative Diabetic Retinopathy
Intraocular Expression of Serum Amyloid A and Interleukin-6 in Proliferative Diabetic Retinopathy
复制标题
增殖性糖尿病视网膜病变中血清淀粉样蛋白 a 和白细胞介素 6 的眼内表达
DOI：
10.1016/j.ajo.2011.03.007
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发表时间：
2011-10-01
影响因子：
4.2
通讯作者：
Jiang, Yan-Rong
中科院分区：
文献类型：
--
作者：
Ma, Yan;Tao, Yong;Jiang, Yan-Rong
目的：由于血清淀粉样蛋白A可以调节血管生成，我们寻找血清淀粉样蛋白A和白细胞介素-6 （IL-6）作为促炎因子与增殖性糖尿病视网膜病变（PDR）之间的关系。设计：回顾性、比较研究。方法：纳入76例（76眼）PDR患者和31例（31眼）非糖尿病性眼病患者（对照组），包括特发性视网膜前膜（8眼）和特发性黄斑孔（23眼）。采用酶联免疫吸附法、双色免疫荧光染色法和半定量反转录聚合酶链反应分别检测玻璃体和血浆中血清淀粉样蛋白A和IL-6水平，以及切除膜中血清淀粉样蛋白A蛋白和mRNA的表达。结果：研究组玻璃体血清淀粉样蛋白A和IL-6水平显著高于对照组（P均< 0.001），而血清淀粉样蛋白A和IL-6血浆浓度在两组间无显著差异（P分别为0.555和0.621）。PDR患者玻璃体中淀粉样蛋白A水平与血浆中淀粉样蛋白A水平呈显著相关（r = 0.525; P < 0.001）。在研究组的纤维血管膜中，内皮标记CD31与血清淀粉样蛋白A共定位，纤维结构标记纤维连接蛋白与血清淀粉样蛋白A共定位。血清淀粉样蛋白A mRNA在PDR纤维血管膜中的表达明显高于特发性视网膜前膜（P = 0.004）。结论：血清淀粉样蛋白A和IL-6可能参与了PDR发生的炎症过程。PDR中可能存在血清淀粉样蛋白A的局部表达。[J]中华眼科杂志，2011;32(2):678-685。(C)爱思唯尔公司2011。版权所有。)
PURPOSE: Because serum amyloid A can regulate angiogenesis, we searched for an association between serum amyloid A and interleukin-6 (IL-6), as proinflammatory factors, and proliferative diabetic retinopathy (PDR).DESIGN: Retrospective, comparative study.METHODS: Seventy-six patients (76 eyes) with PDR and 31 patients (31 eyes) with nondiabetic ocular disease (control group), including idiopathic epiretinal membranes (8 eyes) and idiopathic macular holes (23 eyes), were enrolled. Enzyme-linked immunosorbent assay, dual-color immunofluorescence staining, and semiquantitative reverse-transcription polymerase chain reaction were used to examine the serum amyloid A and IL-6 levels in vitreous and plasma, expression of protein and mRNA of serum amyloid A in the excised membranes, respectively.RESULTS: Vitreous serum amyloid A and IL-6 levels in the study group were significantly higher than those in the control group (both P < .001), whereas the plasma concentrations of serum amyloid A and IL-6 did not vary significantly between the groups (P = .555 and P = .621, respectively). A significant correlation was observed between the vitreous and plasma levels of serum amyloid A in subjects with PDR (r = 0.525; P < .001). In fibrovascular membranes of the study group, colocalization of endothelial marker CD31 with serum amyloid A and colocalization of fibrillar structure markers fibronectin with serum amyloid A were observed. Expression of serum amyloid A mRNA was significantly higher in fibrovascular membranes with PDR than in idiopathic epiretinal membranes (P = .004).CONCLUSIONS: Serum amyloid A and IL-6 may be involved with the inflammatory process in the development of PDR. Local expression of serum amyloid A may exist in PDR. (Am J Ophthalmol 2011;152:678-685. (C) 2011 by Elsevier Inc. All rights reserved.)