Pentalinonsterol, a Constituent of Pentalinon andrieuxii, Possesses Potent Immunomodulatory Activity and Primes T Cell Immune Responses.
Pentalinonsterol, a Constituent of Pentalinon andrieuxii, Possesses Potent Immunomodulatory Activity and Primes T Cell Immune Responses.
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Pentalinonsterol 是 Pentalinon andrieuxii 的成分，具有有效的免疫调节活性并引发 T 细胞免疫反应。
DOI：
10.1021/acs.jnatprod.7b00445
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发表时间：
2017
影响因子：
5.1
通讯作者：
Satoskar,AbhayR
中科院分区：
文献类型：
--
作者：
Oghumu,Steve;Varikuti,Sanjay;Saljoughian,Noushin;Terrazas,Cesar;Huntsman,AndrewC;Parinandi,NarasimhamL;Fuchs,JamesR;Kinghorn,ADouglas;Satoskar,AbhayR
The use of natural products as adjuvants has emerged as a promising approach for the development of effective vaccine formulations. Pentalinonsterol (PEN) is a recently isolated compound from the roots ofPentalinon andrieuxiiand has been shown to possess antileishmanial activity againstLeishmaniaspp. The objective of this study was to examine the immunomodulatory properties of PEN and evaluate its potential as an adjuvant. Macrophages and bone-marrow-derived dendritic cells (BMDCs) were stimulated with PEN and tested for gene expression, cytokine production, and their ability to activate T cells in vitro. PEN was also evaluated for its ability to generate antigen-specific Th1 and Th2 responses in vivo, following ovalbumin (OVA) immunization using PEN as an adjuvant. The results obtained demonstrate that PEN enhances the expression of NF-κB and AP1 transcription factors, promotes gene expression ofTnfα,Il6,Nos2, andArg1, and upregulates MHCII, CD80, and CD86 in macrophages. PEN also enhanced IL-12 production in BMDCs and promoted BMDC-mediated production of IFN-γ by T cells. Further, mice immunized with OVA and PEN showed enhanced antigen-specific Th1 and Th2 cytokines in their splenocytes and lymph node cells, as well as increased levels of IgG1 and IgG2 in their sera. Taken together, this study demonstrates that PEN is a potent immunomodulatory compound and potentially can be used as an adjuvant for vaccine development against infectious diseases.
