Behavior of Primary Cilia and Tricellular Tight Junction Proteins during Differentiation in Temperature-Sensitive Mouse Cochlear Precursor Hair Cells.
Behavior of Primary Cilia and Tricellular Tight Junction Proteins during Differentiation in Temperature-Sensitive Mouse Cochlear Precursor Hair Cells.
复制标题
温度敏感型小鼠耳蜗前体毛细胞分化过程中初级纤毛和三细胞紧密连接蛋白的行为。
DOI：
10.1159/000441867
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Himi T.
中科院分区：
文献类型：
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作者：
Kakuki T;Kaneko Y;Takano K;Ninomiya T;Kohno T;Kojima T;Himi T.
在哺乳动物耳蜗的感觉毛细胞中，平面细胞极性中的初级纤毛以及上皮细胞极性和屏障中的紧密连接对于维持正常听力很重要。使用温度敏感的小鼠耳蜗前体毛细胞来研究初级纤毛和三细胞紧密连接蛋白在感觉毛细胞分化过程中的行为。在未分化细胞（33℃培养）中，观察到许多乙酰化微管蛋白阳性的初级纤毛，并且每个纤毛都伴有γ-微管蛋白阳性的基体。初级纤毛具有“9+0”结构，具有九个外部微管双联体，但缺乏一对中央微管。在分化细胞（39℃培养）中，细胞表面部分观察到乙酰化微管蛋白阳性初级纤毛以及乙酰化微管蛋白阳性纤毛样结构。在分化细胞中，与未分化细胞相比，初级纤毛的数量明显减少，并且在细胞表面部分观察到无数无纤毛袋的纤毛样结构。在未分化细胞中，细胞质中观察到很少的三纤维素分子和脂解刺激脂蛋白受体（LSR）。在分化细胞中，在膜上和细胞质内观察到许多三纤维素分子和LSR。条件永生化小鼠耳蜗前体毛细胞可能有助于研究初级纤毛和三细胞紧密连接在细胞分化和退化（例如细胞凋亡）过程中的作用。© 2016 S. Karger AG，巴塞尔在哺乳动物耳蜗的感觉毛细胞中，初级纤毛在平面细胞极性中的行为以及上皮细胞极性中的紧密连接和 屏障对于细胞分化和分解的开始很重要
In the sensory hair cells of the mammalian cochlea, the primary cilia in the planar cell polarity as well as the tight junctions in the epithelial cell polarity and the barrier are important to maintain normal hearing. Temperature-sensitive mouse cochlear precursor hair cells were used to investigate the behavior of primary cilia and tricellular tight junction proteins during the differentiation of sensory hair cells. In undifferentiated cells (incubated at 33 C), many acetylated tubulin-positive primary cilia were observed, and each was accompanied with an y-tubulin-positive basal body. The primary cilia had a'9+ 0'architecture with nine outer microtubule doublets but lacking a central pair of microtubules. In differenti-ated cells (incubated at 39 C), acetylated tubulin-positive primary cilia as well as acetylated tubulin-positive cilia-like structures were partially observed on the cell surface. In differentiated cells, the number of primary cilia was markedly reduced compared with undifferentiated cells, and innumerable cilia-like structures with no ciliary pock-ets were partially observed on the cell surface. In undifferentiated cells, few tricellulin molecules and lipolysisstimulated lipoprotein receptors (LSRs) were observed in the cytoplasm. In differentiated cells, many tricellulin molecules and LSRs were observed on the membranes and within the cytoplasm. Conditional immortalized mouse cochlear precursor hair cells may be useful to in-vestigate the roles of primary cilia and tricellular tight junctions during cellular differentiation and degeneration such as apoptosis.© 2016 S. Karger AG, BaselIn the sensory hair cells of the mammalian cochlea, the behavior of the primary cilia in the planar cell polarity and the tight junctions in the epithelial cell polarity and the barrier are important for cellular differentiation and the onset of dis-