Endothelial and vascular smooth muscle cell function on poly(lactic-co-glycolic acid) with nano-structured surface features
Endothelial and vascular smooth muscle cell function on poly(lactic-co-glycolic acid) with nano-structured surface features
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DOI：
10.1016/s0142-9612(03)00471-x
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发表时间：
2004-01-01
期刊：
影响因子：
14
通讯作者：
Webster, TJ
中科院分区：
文献类型：
--
作者：
Miller, DC;Thapa, A;Webster, TJ
成功整合到周围组织中的生物材料不仅应匹配组织的机械特性，还应匹配其形貌。在合成聚合物制剂中，通过模仿由天然组织的相关纳米结构的细胞外基质组分产生的表面粗糙度，可以增强对生物材料的细胞反应。作为实现这一奋进的第一步，本体外研究的目标是使用这些设计参数来开发一种合成的、纳米结构的、聚合物生物材料，其促进血管应用的细胞粘附和生长。以一种新的方式，聚（乳酸-共-乙醇酸）（PLGA）（50/50wt%的混合物）的合成具有范围（从微米到纳米）的表面特征。通过用各种浓度的NaOH处理常规PLGA选择的时间段来实现表面特征的减少。细胞实验结果表明，与常规PLGA相比，NaOH处理的PLGA增强了血管平滑肌细胞的粘附和增殖。然而，通过浸泡在NaOH中制备的PLGA与常规PLGA相比降低了内皮细胞的粘附和增殖。经过进一步调查，这一发现被确定为聚合物合成过程中化学（而非形貌）变化的结果。表面化学效应被删除，同时保留纳米结构的地形，通过使用聚合物/弹性体铸造方法。结果表明，内皮细胞和平滑肌细胞密度增加的纳米结构铸造PLGA。由于这些原因，目前的体外研究提供了第一个证据表明，纳米结构的表面特征可以显着提高血管细胞密度;这种设计标准可以用于合成下一代更成功的组织工程血管移植物。(C)2003 Elsevier Ltd.保留所有权利。
Biomaterials that successfully integrate into surrounding tissue should match not only the tissue's mechanical properties, but also its topography. The cellular response to a biomaterial may be enhanced in synthetic polymer formulations by mimicking the surface roughness created by the associated nano-structured extra-cellular matrix components of natural tissue. As a first step towards this endeavor, the goal of the present in vitro study was to use these design parameters to develop a synthetic, nano-structured, polymeric biomaterial that promotes cell adhesion and growth for vascular applications. In a novel manner, poly(lactic-co-glycolic acid) (PLGA) (50/50wt% mix) was synthesized to possess a range (from micron to nanometer) of surface features. Reduction of surface features was accomplished by treating conventional PLGA with various concentrations of NaOH for select periods of time. Results from cell experiments indicated that, compared to conventional PLGA, NaOH treated PLGA enhanced vascular smooth muscle cell adhesion and proliferation. However, PLGA prepared by soaking in NaOH decreased endothelial cell adhesion and proliferation compared to conventional PLGA. After further investigation, this finding was determined to be a result of chemical (and not topographical) changes during polymer synthesis. Surface chemistry effects were removed while retaining nano-structured topography by using polymer/elastomer casting methods. Results demonstrated that endothelial and smooth muscle cell densities increased on nano-structured cast PLGA. For these reasons, the present in vitro study provided the first evidence that nano-structured surface features can significantly improve vascular cell densities; such design criteria can be used in the synthesis of the next-generation of more successful tissue-engineered vascular grafts. (C) 2003 Elsevier Ltd. All rights reserved.