Comprehensive Characteristics of MicroRNA Expression Profile Conferring to Rhizoctonia solani in Rice
Comprehensive Characteristics of MicroRNA Expression Profile Conferring to Rhizoctonia solani in Rice
复制标题
水稻立枯丝核菌 microRNA 表达谱的综合特征
DOI：
10.1016/j.rsci.2019.04.007
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发表时间：
2020-03-01
期刊：
影响因子：
4.8
通讯作者：
Zuo Shimin
中科院分区：
文献类型：
--
作者：
Cao Wenlei;Cao Xinxin;Zuo Shimin
MicroRNA（miRNAs）是一类长度约为22个核苷酸的非编码RNA，在植物对生物和非生物胁迫的反应中起着重要的调控作用。然而，miRNAs是否赋予水稻对坏死营养型真菌立枯丝核菌的抗性仍然是未知的。目的探讨miRNAs是否调控了耐药基因R. Solani的方法，我们构建了12个分别在接种后5 h（hpi）、10 hpi和20 hpi用水/病原体处理的感病和抗病水稻品种的小RNA文库。利用新一代测序技术，我们从文库中共收集到400-450个已知的miRNAs和450-620个新的miRNAs。miRNAs的表达分析表明，已知的和新的miRNAs在R.索拉尼挑战目前已鉴定出34个在水稻中特异表达的miRNA家族，其中大部分与植物抗病性有关。京都基因与基因组百科全书（KEGG）的一个特别的途径分析结果显示，绝大多数被调控的miRNAs的靶基因属于植物-病原体互作途径。此外，miR444b.2、miR 531 a、miR 1861 i、novel_miR1956和novel_miR135赋予对R.通过北方印迹证实了Solani感染。我们对miRNA谱的全面了解表明，miRNA的调控可能与水稻对R.索拉尼对miRNAs表达的分析将为制定防治水稻纹枯病的策略提供指导。
MicroRNAs (miRNAs) are about 22 nucleotides regulatory non-coding RNAs that play versatile roles in reprogramming plant responses to biotic and abiotic stresses. However, it remains unknown whether miRNAs confer the resistance to necrotrophic fungus Rhizoctonia solani in rice. To investigate whether miRNAs regulate the resistance to R. solani, we constructed 12 small RNA libraries from susceptible and resistant rice cultivars treated with water/pathogen at 5 h post inoculation (hpi), 10 hpi and 20 hpi, respectively. By taking the advantage of next-generation sequencing, we totally collected 400-450 known miRNAs and 450-620 novel miRNAs from the libraries. Expression analysis of miRNAs demonstrated different patterns for known and novel miRNAs upon R. solani challenge. Thirty-four miRNA families were identified to be expressed specifically in rice, and most of them were involved in plant disease resistance. A particular Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis result revealed that a great majority of target genes of regulated miRNAs belonged to the pathway of plant-pathogen interaction. Moreover, miR444b.2, miR531a, mir1861i, novel_miR1956 and novel_miR135 conferred response to R. solani infection confirmed by Northern blot. Our global understanding of miRNA profiling revealed that the regulation of miRNAs may be implicated in the control of rice immunity to R. solani. Analysis of the expression of miRNAs will offer the community with a direction to generate appropriate strategies for controlling rice sheath blight disease.