Moving Liquids with Sound: The Physics of Acoustic Droplet Ejection for Robust Laboratory Automation in Life Sciences
Moving Liquids with Sound: The Physics of Acoustic Droplet Ejection for Robust Laboratory Automation in Life Sciences
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DOI：
10.1177/2211068215615096
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发表时间：
2016-02-01
期刊：
影响因子：
--
通讯作者：
Ellson, Richard
中科院分区：
文献类型：
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作者：
Hadimioglu, Babur;Stearns, Richard;Ellson, Richard
用于生命科学应用的液体处理仪器是在十多年前推出的，该仪器基于聚焦声能形成液滴或声液滴喷射（ADE）。虽然用声音移动液体的想法在20世纪就已经为人所知，但在21世纪，随着高密度微孔板上的液滴按需控制声学转移的适应，在实验室中，声学分配到相同生命科学材料的精确方法的发展才真正开始，用于高通量筛选（HTS）应用。用于生命科学应用的强大的ADE实现通过首先使用声学来感知与其转移相关的液体特性，然后通过定制的声能应用于微孔板中的给定井和井流体来驱动液体的转移，从而实现出色的准确性和精度。本文概述了ADE背后的物理原理，以及它在生命科学实验室中强大实施ADE的声学和流变学方面的核心作用，以及它广泛的可弹射材料。
Liquid handling instruments for life science applications based on droplet formation with focused acoustic energy or acoustic droplet ejection (ADE) were introduced commercially more than a decade ago. While the idea of moving liquids with sound was known in the 20th century, the development of precise methods for acoustic dispensing to aliquot life science materials in the laboratory began in earnest in the 21st century with the adaptation of the controlled drop on demand acoustic transfer of droplets from high-density microplates for high-throughput screening (HTS) applications. Robust ADE implementations for life science applications achieve excellent accuracy and precision by using acoustics first to sense the liquid characteristics relevant for its transfer, and then to actuate transfer of the liquid with customized application of sound energy to the given well and well fluid in the microplate. This article provides an overview of the physics behind ADE and its central role in both acoustical and rheological aspects of robust implementation of ADE in the life science laboratory and its broad range of ejectable materials.