Flurothyl seizure susceptibility in rats following prenatal methylazoxymethanol treatment
Flurothyl seizure susceptibility in rats following prenatal methylazoxymethanol treatment
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DOI：
10.1016/0920-1211(95)00094-1
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发表时间：
1996-04-01
影响因子：
2.2
通讯作者：
Schwartzkroin, PA
中科院分区：
文献类型：
--
作者：
Baraban, SC;Schwartzkroin, PA
甲基甲氧基甲醇醋酸酯（MAMac）是一种强致畸剂，可在发育中的大鼠脑中产生异位细胞。在本研究中，我们评估了(1)产前暴露于MAMac是否会导致氟氟基发作阈值降低，以及(2)暴露于MAMac的海马中异位细胞数量与氟氟基诱发的发作潜伏期之间是否存在相关性。60日龄（P60）大鼠在子宫内暴露于MAMac后，肌阵挛潜伏期（173 +/- 2.3 s）和前肢阵挛潜伏期（215 +/- 4.6 s）明显短于对照组（200 +/- 6.9 s和238 +/- 8.8 s）。MAMac还增加了氟化氟治疗的大鼠从双侧前肢阵挛发展为全身性强直阵挛发作的比例（对照组：33%；MAMac: 91%）。较短的癫痫发作潜伏期与CA1/CA2区异位锥体细胞数量的增加有关。这些结果表明，癫痫易感性在以异常神经元迁移为特征的动物模型（MAMac）中增强。
Methylazoxymethanol acetate (MAMac) is a potent teratogenic agent which can produce ectopic cell placement in developing rat brains. In the present study, we evaluated (i) whether prenatal exposure to MAMac results in a lowered seizure threshold to flurothyl and (ii) if there is a correlation between the number of ectopic cells in MAMac-exposed hippocampus and flurothyl-induced seizure latency. In 60 day old (P60) rats exposed to MAMac in utero, the latencies to myoclonic jerk (173 +/- 2.3 s) and forelimb clonus (215 +/- 4.6 s) were significantly shorter than those of controls (200 +/- 6.9 s and 238 +/- 8.8 s, respectively). MAMac also increased the proportion of flurothyl-treated rats that progressed from bilateral forelimb clonus to generalized tonic-clonic seizures (control: 33%; MAMac: 91%). Shorter seizure latencies were associated with an increased number of ectopic pyramidal cells in region CA1/CA2. These results suggest seizure susceptibility is enhanced in an animal model (MAMac) characterized by abnormal neuronal migration.