Lipid profiling analyses from mouse models and human infants.
Lipid profiling analyses from mouse models and human infants.
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小鼠模型和人类婴儿的脂质分析分析。
DOI：
10.1016/j.xpro.2022.101679
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发表时间：
2022-12-16
期刊：
影响因子：
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通讯作者：
Koulman, Albert
中科院分区：
文献类型：
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作者：
Olga, Laurentya;Bobeldijk-Pastorova, Ivana;Bas, Richard C;Seidel, Florine;Snowden, Stuart G;Furse, Samuel;Ong, Ken K;Kleemann, Robert;Koulman, Albert
该方案概述了一种转化脂质组学方法，以发现可以预测形态学身体和组织学器官测量的脂质生物标志物（例如，体重和肥胖增加）在生命的特定阶段（例如，早期生活）。我们描述了从动物实验和组织学分析到下游分析步骤的程序，包括小鼠和人类的脂质分析。该方案代表了一种可靠且通用的方法，用于将候选脂质生物标志物从动物模型转化和验证到人类队列。有关本方案使用和执行的完整详细信息，请参阅。开发可翻译的血脂异常/肥胖动物模型以模拟人类生理学在暴露于高脂肪饮食之前/之后在小鼠中的脂质组学以捕获脂质生物标志物验证从小鼠到人类婴儿的脂质生物标志物（反映早期生活背景）对从婴儿足跟穿刺中获得的干血斑进行脂质分析。进行任何实验方案都需要遵守当地实验室安全和伦理的机构指南。该方案概述了一种转化脂质组学方法，以发现可以预测形态学身体和组织学器官测量的脂质生物标志物（例如，体重和肥胖增加）在生命的特定阶段（例如，早期生活）。我们描述了从动物实验和组织学分析到下游分析步骤的程序，包括小鼠和人类的脂质分析。该方案代表了一种可靠且通用的方法，用于将候选脂质生物标志物从动物模型转化和验证到人类队列。
This protocol outlines a translational lipidomic approach to discover lipid biomarkers that could predict morphometric body and histological organ measurements (e.g., weight and adiposity gains) during specific stages of life (e.g., early life). We describe procedures ranging from animal experimentation and histological analyses to downstream analytical steps through lipid profiling, both in mice and humans. This protocol represents a reliable and versatile approach to translate and validate candidate lipid biomarkers from animal models to a human cohort. For complete details on the use and execution of this protocol, please refer to. Development of translatable dyslipidemic/obese animal models to mimic human physiology Lipidomics in mice before/after exposure to high-fat diet to capture lipid biomarkers Validating lipid biomarkers from mice to human infants (reflecting early life context) Lipid profiling analysis on dried blood spots obtained from heel-prick in infants Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. This protocol outlines a translational lipidomic approach to discover lipid biomarkers that could predict morphometric body and histological organ measurements (e.g., weight and adiposity gains) during specific stages of life (e.g., early life). We describe procedures ranging from animal experimentation and histological analyses to downstream analytical steps through lipid profiling, both in mice and humans. This protocol represents a reliable and versatile approach to translate and validate candidate lipid biomarkers from animal models to a human cohort.