Mitotic delay and chromosomal aberrations induced by X rays in synchronized Chinese hamster cells in vitro.
Mitotic delay and chromosomal aberrations induced by X rays in synchronized Chinese hamster cells in vitro.
复制标题
X射线在体外同步中国仓鼠细胞中诱导的有丝分裂延迟和染色体畸变。
DOI：
10.1093/jnci/39.4.619
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发表时间：
1967
期刊：
影响因子：
--
通讯作者：
W. K. Sinclair
中科院分区：
文献类型：
--
作者：
C. Yu;W. K. Sinclair
在细胞周期的不同阶段对体外同步中华仓鼠细胞群进行x射线照射，并在下一个中期进行检测。有丝分裂延迟随辐照时细胞周期所处阶段的不同而变化。在S中后期照射的细胞最大，G2照射的细胞中等，G1照射的细胞最小。随剂量增加（0.7 hr）近似线性增加。S细胞/100 rad， 0.3 hr。/100 rad （G1细胞）。染色体畸变率也取决于细胞辐照的细胞周期阶段。染色单体畸变主要发生在G2和S期（S期早期比S期晚期多），而染色体畸变主要发生在G1期。500 R和750 R后的总断路在S后期出现频率最低，在G2出现频率最高。这种反应形式与先前报道的细胞周期阶段依赖性相关，但仅在一般定性方式上相关。有丝分裂延迟依赖于细胞周期阶段，与染色体畸变或存活完全不同。
Synchronized Chinese hamster cell populations in vitro were X-irradiated at different stages of the cell cycle and examined at the next metaphase. Mitotic delay varied according to the stage of the cell cycle at the time of irradiation. It was greatest for cells irradiated in middle to late S, moderate for cells irradiated in G2, and least for cells irradiated in G1. It increased approximately linearly with dose (0.7 hr./100 rad for S cells and 0.3 hr./100 rad for G1 cells). Chromosomal aberration rates also depended on the stage of the cell cycle at which the cells were irradiated. Chromatid aberrations were induced mostly during G2 and S (more in early S than late S), while chromosome aberrations were induced mainly in G1. Total breaks after 500 or 750 R showed a minimum frequency in late S and a maximum frequency in G2. This form of response correlates, but only in a general qualitative way, with the dependence of survival on cell cycle stage previously reported. Mitotic delay has a dependence on cell cycle stage quite different from either chromosomal aberrations or survival.