Schwann cell-derived neuregulin-2alpha can function as a cell-attached activator of muscle acetylcholine receptor expression.
Schwann cell-derived neuregulin-2alpha can function as a cell-attached activator of muscle acetylcholine receptor expression.
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雪旺细胞衍生的神经调节蛋白-2α 可以作为肌肉乙酰胆碱受体表达的细胞附着激活剂。
作者：
Ponomareva,OlgaN;Fischer,TobiasM;Lai,Cary;Rimer,Mendell
在这里，我们发现神经调节蛋白-2(NRG-2)α-和β-亚型可以作为表面连接的配体激活乙酰胆碱受体(AChR)的转录。更重要的是，我们证明了在其细胞表面表达NRG-2α的雪旺细胞，如果被引入细胞与细胞之间的接触，可以诱导AChR的表达，如果将其与特异性表达ErbB4的肌管接触，则与神经肌肉连接处的终末雪旺细胞表达的NRG-2相同。D6P2T细胞系雪旺细胞和NRG-2β转染组的293T细胞均能明显诱导AChR的表达，NRG-2以可溶性形式存在时AChR诱导活性最高。这些结果为先前报道的NRG-2α在突触周围的矛盾积累提供了潜在的作用，NRG-2AChR是以可溶性形式呈现时诱导活性最低的亚型。他们还提出了一种可能性，即雪旺细胞来源的NRG-2可以激活突触肌膜上的ErbB受体，这可能至少部分解释了NRG介导的AChR表达调节。©2006 Wiley-Liss，Inc.
Here we show that neuregulin‐2 (Nrg‐2) α‐ and β‐isoforms can activate acetylcholine receptor (AChR) transcription as surface‐attached ligands. More importantly, we demonstrate that Schwann cells that express Nrg‐2α on their cell surface, the same Nrg‐2 isoform expressed by terminal Schwann cells at the neuromuscular junction, can induceAChRexpression if brought into cell‐to‐cell contact with myotubes specifically expressing ErbB4. These Schwann cells, the D6P2T cell line, induceAChRexpression apparently as well as 293T cells transfected with Nrg‐2β, the isoform with the highest AChR‐inducing activity when presented in a soluble form. These results provide a potential role for the previously reported, paradoxical perisynaptic accumulation of Nrg‐2α, the isoform with the least AChR‐inducing activity when presented in a soluble form. They also raise the possibility that Schwann cell‐derived Nrg‐2 could activate ErbB receptors on the synaptic sarcolemma and that this could account, at least in part, for the Nrg‐mediated regulation ofAChRexpression. © 2006 Wiley‐Liss, Inc.