Beta-endorphin in serum and seminal plasma in infertile men.
Beta-endorphin in serum and seminal plasma in infertile men.
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DOI：
10.1111/j.1745-7262.2006.00180.x
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发表时间：
2006-11
影响因子：
2.9
通讯作者：
S. El-Haggar;S. El-Ashmawy;A. Attia;T. Mostafa;M. Roaiah;A. Fayez;S. Ghazi;W. Zohdy;N. Roshdy
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文献类型：
--
作者：
S. El-Haggar;S. El-Ashmawy;A. Attia;T. Mostafa;M. Roaiah;A. Fayez;S. Ghazi;W. Zohdy;N. Roshdy
目的了解不同不育男性血清和精浆中β-内啡肽水平。方法采用酶联免疫吸附试验(EL ISA)检测80例男性不育症患者血清和精浆中β-内啡肽的含量，并将其分为4组：非梗阻性无精子症(NOA)、梗阻性无精子症(OA)、先天性双侧输精管缺如(CBVAD)和弱精子症。这一结果与20名正常精子症已被证实有生育能力的男性进行了比较。结果连续不育组(NOA51.30+/-27.37，OA51.88+/-9.47，CBAVD20.36+/-13.39，弱精子症49.26+/-12.49pg/mL)精浆β-内啡肽水平均低于正常生育对照组(87.23+/-29.55pg/mL)。4个不育组(分别为51.09+/-14.71、49.76+/-12.4、33.96+/-7.2、69.1+/-16.57pg/m L)与正常生育组(49.26+/-31.32 pg/m L)之间的血清β-内啡肽水平无相关性。CBVAD组精浆中β-内啡肽的平均水平最低。精液中的β-内啡肽对睾丸的贡献估计约为40%。精液β-内啡肽与精子密度呈显著正相关(r=0.699，P=0.0188)，与血清水平(r=0.375，P=0.185)及精子活动率(r=0.470，P=0.899)无显著相关。结论单独测定β-内啡肽对男性生殖功能的评估并不是决定性的，因为还有许多其他阿片类药物作用于下丘脑-垂体性腺轴。
AIM To access beta-endorphin levels in serum as well as seminal plasma in different infertile male groups. METHODS Beta-endorphin was estimated in the serum and seminal plasma by enzyme-linked immunosorbent assay (ELISA) method in 80 infertile men equally divided into four groups: non-obstructive azoospermia (NOA), obstructive azoospermia (OA), congenital bilateral absent vas deferens (CBVAD) and asthenozoospermia. The results were compared to those of 20 normozoospermic proven fertile men. RESULTS There was a decrease in the mean levels of beta-endorphin in the seminal plasma of all successive infertile groups (mean +/- SD: NOA 51.30 +/- 27.37, OA 51.88 +/- 9.47, CBAVD 20.36 +/- 13.39, asthenozoospermia 49.26 +/- 12.49 pg/mL, respectively) compared to the normozoospermic fertile control (87.23 +/- 29.55 pg/mL). This relation was not present in mean serum level of beta-endorphin between four infertile groups (51.09 +/- 14.71, 49.76 +/- 12.4, 33.96 +/- 7.2, 69.1 +/- 16.57 pg/mL, respectively) and the fertile control group (49.26 +/- 31.32 pg/mL). The CBVAD group showed the lowest seminal plasma mean level of beta-endorphin. Testicular contribution of seminal beta-endorphin was estimated to be approximately 40%. Seminal beta-endorphin showed significant correlation with the sperm concentration (r = 0.699, P = 0.0188) and nonsignificant correlation with its serum level (r = 0.375, P = 0.185) or with the sperm motility percentage (r = 0.470, P = 0.899). CONCLUSION The estimation of beta-endorphin alone is not conclusive to evaluate male reproduction as there are many other opiates acting at the hypothalamic pituitary gonadal axis.