Effect of curing time and concentration for a chemical treatment that improves surface gliding for extrasynovial tendon grafts in vitro.
Effect of curing time and concentration for a chemical treatment that improves surface gliding for extrasynovial tendon grafts in vitro.
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化学处理的固化时间和浓度对改善体外滑膜外肌腱移植物表面滑动的影响。
DOI：
10.1002/jbm.a.30868
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
Amadio,PeterC
中科院分区：
文献类型：
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作者：
Tanaka,Toshikazu;Sun,Yu-Long;Zhao,Chunfeng;Zobitz,MarkE;An,Kai-Nan;Amadio,PeterC
这项研究的目的是评估这些试剂的处理时间和浓度是否对所产生的滑行阻力产生影响。选用20只成年杂种犬的40条腓骨长肌腱和取自同侧后爪的A2滑车。分别用透明质酸(HA)和1-乙基-3-[3-二甲氨基丙基]碳二亚胺(EDC)或N-羟基琥珀酰亚胺(NHS)与10%明胶混合5、30、60min。在1000个模拟的屈/伸运动周期中测量肌腱摩擦力。未处理的PL肌腱的抗滑移能力在各组间无显著差异。在低浓度HA和EDC/NHS表面处理5min后，其抗滑性与未经处理的PL肌腱相似，显著高于其处理30min和60min。其余高浓度HA组和EDC/NHS组的抗滑性均低于未经处理的PL腱。然而，不同时间点之间没有显著差异。在犬体外滑膜外肌腱中，通过调节固化时间和试剂浓度，可以优化表面处理对摩擦力和耐久性的影响。©2006 Wiley期刊，Inc.生物材料研究杂志，2006
The purpose of this study was to evaluate whether treatment time and concentration of these reagents have an effect on the resulting gliding resistance. Forty peroneus longus (PL) tendons were used, from 20 adult mongrel dogs, along with the A2 pulley obtained from the ipsilateral hind paw. After the baseline gliding resistance was measured, the PL tendons were treated with one of three concentrations of hyaluronic acid (HA) and 1‐ethyl‐3‐[3‐dimethylaminopropyl] carbodiimide hydrochloride (EDC) orN‐hydroxysuccinimide (NHS) mixed with 10% gelatin for various times (5, 30, and 60 min). Tendon friction was measured over 1000 cycles of simulated flexion/extension motion. Gliding resistance of the untreated PL tendons had no significant difference among the groups. After surface treatment with low concentration of HA and EDC/NHS for 5‐min cure, the gliding resistance was similar to that of the untreated PL tendon and significantly higher than its 30‐ and 60‐min treatment. For the rest of high concentration of HA and EDC/NHS groups, the gliding resistance was lower than that of untreated PL tendon. However, there was no significant difference among the timing points. It is possible to optimize the effect of surface treatment on friction and durability by regulating cure time and concentration of reagents in a canine extrasynovial tendonin vitro. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006