Disk diffusion‐based method aids in the detection of vanM‐positive Enterococcus faecium with low vancomycin minimum inhibitory concentrations
Disk diffusion‐based method aids in the detection of vanM‐positive Enterococcus faecium with low vancomycin minimum inhibitory concentrations
复制标题
DOI：
10.1111/1440-1681.13772
复制
发表时间：
2023-03
影响因子：
2.9
通讯作者：
Jue Wang;Pei Li;Ling Xin;Xiaogang Xu;Minggui Wang
中科院分区：
文献类型：
--
作者：
Jue Wang;Pei Li;Ling Xin;Xiaogang Xu;Minggui Wang
中国于2006年首次报道了上海地区肠球菌中的万古霉素耐药基因，后来发现它是耐万古霉素肠球菌的主要vA基因。本研究连续收集复旦大学华山医院门诊和住院患者粪肠球菌1292株，经VITEK-2系统检测，几乎所有分离株(1290/1292)对万古霉素敏感。然而，采用改良的纸片扩散法，10株先前被VITEK-2系统确定为万古霉素敏感的粪肠球菌被发现在万古霉素纸片抑制区有菌落。脉冲场凝胶电泳结果表明，抑制区的每个随机选择的菌落与原始菌株属于同一克隆。所有10个分离株后来都被发现是VanM阳性。纸片扩散法可用于检测万古霉素最低抑菌浓度较低的VANM阳性粪肠球菌，并可防止对万古霉素敏感变异性肠球菌的漏检。
We first reported the vanM vancomycin resistance gene in enterococci in Shanghai, China in 2006 and later found it to be the predominant van gene in vancomycin‐resistant enterococci (VRE). In this study, we successively collected 1292 Enterococcus faecium and Enterococcus faecalis strains from in‐ and outpatients at Huashan Hospital, Fudan University and found that nearly all of the isolates (1290/1292) were vancomycin‐sensitive determined by the VITEK 2 system. However, using a modified macromethod‐based disk diffusion test, 10 E. faecium isolates that were previously determined to be vancomycin‐sensitive by the VITEK 2 system were found to have colonies in the vancomycin disk inhibition zone. Pulse‐field gel electrophoresis results showed that each randomly selected colony in the inhibition zone belonged to the same clone as the original strain. All 10 isolates were later found to be vanM‐positive. The disk diffusion‐based method may aid in the detection of vanM‐positive E. faecium with low vancomycin minimum inhibitory concentrations and prevent missing the detection of vancomycin sensitivity‐variable enterococci.