No immunogenicity of IPS cells in syngeneic host studied by in vivo injection and 3D scaffold experiments.
No immunogenicity of IPS cells in syngeneic host studied by in vivo injection and 3D scaffold experiments.
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DOI：
10.1155/2013/378207
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发表时间：
2013
影响因子：
--
通讯作者：
Isobe K
中科院分区：
文献类型：
--
作者：
Thanasegaran S;Cheng Z;Ito S;Nishio N;Isobe K
诱导多能干细胞（IPSCs）为利用患者自身组织治疗以前无法治愈的疾病提供了极大的可能性。然而，这些细胞只有在移植回同基因宿主时不被排斥的情况下才能用于细胞治疗。我们发现，将来自不同年龄小鼠的iPSC注射到同基因C57 BL/6小鼠中产生畸胎瘤，并且没有被排斥。将诱导分化后的iPSCs和髓样分化的iPSCs分别在三维多孔支架中培养，并移植到C57 BL/6小鼠和BALB/C小鼠体内。移植后，我们可以观察到与同种异体小鼠相比，同基因小鼠支架内的细胞密度迅速增加，表明支持iPSC体内生长的有利条件。与同种异体对应物不同，我们在同基因小鼠的支架内没有观察到少量浸润的T细胞。这些结果有助于在不久的将来对iPSCs用于再生医学的乐观看法。
Induced Pluripotent Stem Cells (IPSCs) open the great possibility to employ patient's own tissue to the previously incurable diseases. However these cells can be used in cell therapy only if they are not rejected when transplanted back into the syngeneic host. We found that the injection of iPSCs derived from different ages of mice into syngeneic C57BL/6 mice produced teratoma and was not rejected. Then we cultured iPSCs and myeloid differentiated iPSCs in three-dimensional porous scaffold and transplanted to C57BL/6 mice and BALB/C mice. After transplantation, we could observe the cell density inside the scaffold increased rapidly in syngeneic mice compared to the allogeneic mice indicating the favorable conditions supporting the growth of iPSCs in vivo. Unlike the allogeneic counterpart, we could not observe few infiltrating T cells inside the scaffold of syngeneic mice. These results contribute to the optimistic view of iPSCs for regenerative medicine in near future.
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影响因子：
3.7
作者：
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通讯作者：
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作者：
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作者：
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