Expression and clinical significance of SALL4 and LGR5 in patients with lung cancer
Expression and clinical significance of SALL4 and LGR5 in patients with lung cancer
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DOI：
10.3892/ol.2015.3772
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发表时间：
2015-12-01
期刊：
影响因子：
2.9
通讯作者：
Mo, Biwen
中科院分区：
文献类型：
--
作者：
Gautam, Ajay Kumar;Wang, Changming;Mo, Biwen
就发病率和死亡率而言，肺癌是全世界最常见的癌症。由于疾病诊断的挑战，5年总生存率仅为16%。以前的研究表明，恶性转化起源于成体干细胞，因此恶性病变可能表达干细胞相关标志物。本研究旨在探讨干细胞相关标志物Sal样蛋白4（SALL 4）和富含亮氨酸重复序列的G蛋白偶联受体5（LGR 5）在肺癌中的表达及其临床意义，为肺癌的诊断和治疗提供新的标志物和靶点。通过对135例人肺癌组织标本和10例非癌肺组织标本进行免疫组化分析干细胞相关标志物SALL 4和LGR 5的表达。还评估了这些标志物表达的临床意义及其表达与临床参数之间的相关性。SALL4在肺癌组织中表达显著上调，而在非癌肺组织中不表达，其敏感性和特异性分别为88%和100%。相比之下，LGR 5表现出97%的敏感性，但特异性差。因此，SALL 4可能是肺癌的一个非常有用的诊断标志物，但LGR 5不是那么有用。
Lung cancer is the most frequent cancer worldwide, in terms of incidence and mortality. Due to challenges in the diagnosis of the disease, the 5-year overall survival rate is only similar to 16%. Previous studies have suggested that malignant transformations originate from adult stem cells, and malignant lesions may therefore express stem-cell-associated markers. The purpose of the present study is to investigate the expression and clinical significance of the stem cell-associated markers Sal-like protein 4 (SALL4) and leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5) in lung cancer, and to provide novel diagnostic markers and targets for the treatment of lung cancer. The expression of the stem cell-associated markers SALL4 and LGR5 was analyzed by immunohistochemistry performed on 135 human lung cancer tissue specimens and 10 non-cancer lung tissue specimens. The clinical significance of the expression of these markers and correlation between their expression and clinical parameters was also assessed. SALL4 expression was highly upregulated in lung cancer tissues, but was not present in non-cancerous lung tissues, and the sensitivity and specificity of SALL4 reached 88% and 100%, respectively. By contrast, LGR5 demonstrated 97% sensitivity, but the specificity was poor. Therefore, SALL4 may be an extremely useful diagnostic marker for lung cancer, but LGR5 is not as useful.