Expression of Wnt gene family and frizzled receptors in head and neck squamous cell carcinomas
Expression of Wnt gene family and frizzled receptors in head and neck squamous cell carcinomas
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DOI：
10.1007/s00428-009-0793-z
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发表时间：
2009-07-01
期刊：
影响因子：
3.5
通讯作者：
Anton Aparicio, Luis M.
中科院分区：
文献类型：
--
作者：
Diaz Prado, Silvia Maria;Medina Villaamil, Vanessa;Anton Aparicio, Luis M.
在HNSCC组织和细胞系中表达的Wnt和Frizzled类基因在细胞形态发生和分化中具有既定的作用，并且它们还具有致癌特性。我们通过qPCR研究了Wnt和Fz基因作为HNSCC中潜在的肿瘤相关标志物。检测了22例HNSCC冷冻标本中Wnt和Fz基因的表达水平。我们还评估了在癌症样本中获得的表达水平与临床病理结果之间的可能相关性。Wnt-1在大多数研究的HNSCC中不表达，而Wnt-5A在恶性肿瘤中表达最强。Wnt-10B表达水平与未分化程度有关。与Fz基因相关，Fz-5在未受影响的区域淋巴结中表现出更多的表达水平。Kaplan-Meier生存分析表明Wnt-7A和Fz-5 mRNA的低表达和高表达分别缩短了生存时间。qPCR证实HNSCC表达Wnt和Fz成员，并表明Wnt和Fz信号在HNSCC细胞中被激活。
Genes of the Wnt and Frizzled class, expressed in HNSCC tissue and cell lines, have an established role in cell morphogenesis and differentiation, and also they have oncogenic properties. We studied Wnt and Fz genes as potential tumor-associated markers in HNSCC by qPCR. Expression levels of Wnt and Fz genes in 22 unique frozen samples from HNSCC were measured. We also assessed possible correlation between the expression levels obtained in cancer samples in relation to clinicopathologic outcome. Wnt-1 was not expressed in the majority of the HNSCC studied, whereas Wnt-5A was the most strongly expressed by the malignant tumors. Wnt-10B expression levels were related with higher grade of undifferentiation. Related to Fz genes, Fz-5 showed more expression levels in no-affectation of regional lymph nodes. Kaplan-Meier survival analyses suggest a reduced time of survival for low and high expression of Wnt-7A and Fz-5 mRNA, respectively. qPCR demonstrated that HNSCC express Wnt and Fz members, and suggested that Wnt and Fz signaling is activated in HNSCC cells.