Metabolism of platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) in human fetal membranes and decidua vera.
Metabolism of platelet-activating factor (1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) in human fetal membranes and decidua vera.
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人胎膜和蜕膜中血小板激活因子（1-O-烷基-2-乙酰基-sn-甘油-3-磷酸胆碱）的代谢。
DOI：
10.1016/0003-9861(86)90444-3
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发表时间：
1986
影响因子：
3.9
通讯作者：
Johnston,JM
中科院分区：
文献类型：
--
作者：
Ban,C;Billah,MM;Truong,CT;Johnston,JM
我们以前曾报道，血小板活化因子（PAF）是目前在人类羊水中获得的妇女在劳动。我们还证实了PAF、溶血PAF和烷基酰基-sn-甘油-3-磷酸胆碱（AA-GPC）存在于人羊膜组织中。在本研究中，我们研究了羊膜组织中PAF代谢的相关酶及其调控。在羊膜组织中已证实磷脂酶A2具有裂解烷基酰基（长链）sn-甘油-3-磷酸胆碱的活性。酶活性不改变Ca 2+，是明显不同的磷脂酶A2，我们以前在这个组织中的特点。羊膜组织含有乙酰转移酶活性，其需要Ca 2+并与微粒体部分相关。乙酰水解酶也存在于羊膜组织的胞质组分中。乙酰水解酶活性也已在羊水中得到证实。乙酰转移酶（溶PAF）和乙酰水解酶（PAF）的亲和力不受Ca ~（2+）。然而，在Ca 2+的存在下，乙酰转移酶的比活性增加4 - 5倍，而乙酰水解酶不受影响。胎膜和蜕膜中的乙酰转移酶和乙酰水解酶活性相似，且不随孕周变化。PAF在人类分娩的启动可能发挥的作用进行了讨论。
We have previously reported that platelet-activating factor (PAF) is present in human amniotic fluid obtained from women in labor. We have also demonstrated that PAF, lyso-PAF, and alkyl acyl-sn-glycero-3-phosphocholine (AA-GPC) are present in human amnion tissue. In the reported study, we have investigated the enzymes involved in PAF metabolism in amnion tissue and their regulation. A phospholipase A2activity has been demonstrated in amnion tissue which cleaves alkyl acyl (long-chain)sn-glycero-3-phosphocholine. The enzyme activity is not altered by Ca2+and is distinctly different from the phospholipase A2that we have previously characterized in this tissue. Amnion tissue contains acetyltransferase activity which requires Ca2+and is associated with the microsomal fraction. Acetylhydrolase is also present in the cytosolic fraction of amnion tissue. Acetylhydrolase activity has also been demonstrated in amniotic fluid. The affinities of acetyltransferase (for lyso-PAF) and acetylhydrolase (for PAF) were unaffected by Ca2+. In the presence of Ca2+, however, the specific activity of acetyltransferase was increased four- to fivefold while that of acetylhydrolase was unaffected. Acetyltransferase and acetylhydrolase activities in fetal membranes and decidua were similar and were unchanged with gestational age. The possible role of PAF in the initiation of human parturition is discussed.