Effects of dietary Saccharomyces cerevisiae fermentation product (DVAQUA®) on growth performance, intestinal autochthonous bacterial community and non-specific immunity of hybrid tilapia (Oreochromis niloticus ♀xO. aureus ♂) cultured in cages
Effects of dietary Saccharomyces cerevisiae fermentation product (DVAQUA®) on growth performance, intestinal autochthonous bacterial community and non-specific immunity of hybrid tilapia (Oreochromis niloticus ♀xO. aureus ♂) cultured in cages
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DOI：
10.1016/j.aquaculture.2009.04.043
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发表时间：
2009-09-01
期刊：
影响因子：
4.5
通讯作者：
Yoon, Ilkyu
中科院分区：
文献类型：
--
作者：
He, Suxu;Zhou, Zhigang;Yoon, Ilkyu
本研究以尼罗罗非鱼（Oreochromis niloticus female x O.金黄色葡萄球菌雄性）在笼中饲喂六种等氮和缺血的实验饮食。日粮中添加6个水平的酿酒酵母发酵产物（DVAQU（R））：0（CK）。0.125（G1）、0.25（G2）、0.5（0）、1.0（G4）和2.0（G5）g kg（-1）日粮。本研究旨在探讨饲料中添加DVAQUA对罗非鱼生长性能、肠道细菌群落结构和非特异性免疫功能的影响。饲料中添加DVAQUA对罗非鱼的生长性能、饲料转化率和成活率没有影响。然而，补充DVAQUA影响了本地肠道细菌群落。在不同的饲养阶段，饲粮DVAQUA对各种潜在的有益菌均有刺激作用，而对潜在的有害菌如大肠杆菌020：H42样、未培养的芽孢杆菌M5030 A1-F02样和荧光假单胞菌YC 0357样均有抑制作用。DVAQUA可提高罗非鱼血清溶菌酶活性、补体旁路途径（C3和C4）活性、头肾巨噬细胞吞噬活性和头肾巨噬细胞呼吸爆发活性等非特异性免疫功能。用折线法确定的DVAQUA对罗非鱼的最适添加量，对溶菌酶、C3、C4和吞噬细胞活性的影响分别为0.226、0.467、0.492和0.50 g/kg。(C)2009爱思唯尔有限公司版权所有。
In the present study, hybrid tilapias (Oreochromis niloticus female x O. aureus male) were fed six isonitrogenous and iscrenergetic experimental diets in cages. The diets contained six levels of Saccharomyces cerevisiae fermentation product (DVAQU (R)): 0 (CK). 0.125 (G1), 0.25 (G2),0.5 (0), 1.0 (G4) and 2.0 (G5) g kg(-1) diet. The purpose of this study was to evaluate the effects of dietary DVAQUA on growth performance, intestinal autochthonous bacterial community by using 16S rDNA and denaturing gradient gel electrophoresis (DGGE), and non-specific immunity of hybrid tilapia. Supplementation of dietary DVAQUA showed no effects on growth performance, diet conversion and survival rate of the hybrid tilapia. However, supplementation of DVAQUA affected the autochthonous gut bacteria community. Various potentially beneficial bacteria were stimulated by dietary DVAQUA at different feeding period, while potential harmful species such as Escherichia coli serotype 020: H42-like, uncultured Bacilli bacterium clone M5030A1-F02-like, and Pseudomonas fluorescens strain YC0357-like were depressed by dietary DVAQUA. Non-specific immunity of hybrid tilapia such as serum lysozyme activity, serum alternative complement pathway (C3 and C4) activities, head kidney macrophage phagocytic activity, and head kidney macrophage respiratory burst activity were improved by dietary DVAQUA. The optimum dose of dietary DVAQUA for hybrid tilapia determined based on a broken-line approach varies depending on the variables used: 0.226, 0.467,0.492 or 0.50 g kg(-1) diet for lysozyme, C3, C4, or phagocytic activity, respectively. (C) 2009 Elsevier B.V. All rights reserved.