Directing nuclear deformation on micropillared surfaces by substrate geometry and cytoskeleton organization
Directing nuclear deformation on micropillared surfaces by substrate geometry and cytoskeleton organization
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DOI：
10.1016/j.biomaterials.2013.01.018
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发表时间：
2013-04-01
期刊：
影响因子：
14
通讯作者：
Anselme, Karine
中科院分区：
文献类型：
--
作者：
Badique, Florent;Stamov, Dimitar R.;Anselme, Karine
我们最近已经证明了SaOs-2骨肉瘤细胞在poly-I上的强烈核变形。乳酸（PLLA）微柱基底。在本研究中，我们首先证明了微柱基底的化学和机械性能对变形没有主导作用。然而，SaOs-2核变形可以通过改变柱尺寸和间距来强烈调制，突出了几何约束对核成形的重要性。此外，比较三种不同的骨肉瘤细胞系（SaOs-2，MG-63和OHS-4）的核变形能力，显示出强烈的细胞类型特异性差异。令人惊讶的是，高度可变形的SaOs-2细胞系显示出最高的细胞刚度，如通过基于AFM的胶体力光谱法所评估的，并且在细胞核上方具有更突出的肌动蛋白纤维阵列，这表明肌动蛋白介导的细胞刚度和细胞核变形之间存在联系。相比之下，在MG-63和OHS-4细胞中，即使在没有突出的肌动蛋白细胞骨架的情况下，致密的微管和波形蛋白网络似乎也有助于一些核变形。总之，这些结果表明，所有三个细胞骨架元素的相互作用是需要有效的核变形。总之，影响微柱基底上核变形的主要参数不是它们的材料特性，而是基底几何形状以及细胞表型和细胞骨架组织。(C)2013爱思唯尔有限公司保留所有权利。
We have recently demonstrated strong nuclear deformation of SaOs-2 osteosarcoma cells on poly-I.-lactic acid (PLLA) micropillar substrates. In the present study, we first demonstrated that chemical and mechanical properties of the micropillar substrates have no dominant effect on deformation. However, SaOs-2 nucleus deformation could be strongly modulated by varying the pillar size and spacing, highlighting the importance of geometric constraints for shaping the nucleus. Furthermore, comparing the capacity for nuclear deformation in three different osteosarcoma cell lines (SaOs-2, MG-63 and OHS-4) revealed strong cell-type specific differences. Surprisingly, the highly-deformable SaOs-2 cell line displayed the highest cell stiffness as assessed by AFM-based colloidal force spectroscopy and featured a more prominent array of actin fibres above the nucleus, suggesting a link between actin-mediated cell stiffness and cell nucleus deformation. In contrast, in MG-63 and OHS-4 cells dense microtubule and vimentin networks seem to facilitate some nuclear deformation even in the absence of a prominent actin cytoskeleton. Together these results suggest that an interaction of all three cytoskeletal elements is needed for efficient nuclear deformation. In conclusion, the dominant parameters influencing nuclear deformation on micropillar substrates are not their material properties but the substrate geometry together with cell phenotype and cytoskeleton organization. (C) 2013 Elsevier Ltd. All rights reserved.