Postnatal maturation of renal cortical peritubular fibroblasts in the rat
Postnatal maturation of renal cortical peritubular fibroblasts in the rat
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DOI：
10.1007/s004290050126
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发表时间：
1998-02-01
期刊：
影响因子：
--
通讯作者：
Kaissling, B
中科院分区：
文献类型：
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作者：
Marxer-Meier, A;Hegyi, I;Kaissling, B
成年大鼠肾皮质和髓质的基质细胞表现出不同的表型。皮质管周成纤维细胞是外-5 '核苷酸酶（5' NT）阳性，缺乏α-平滑肌肌动蛋白（α SMA）和波形蛋白免疫反应性，而髓质成纤维细胞是5 'NT阴性和波形蛋白阳性。我们已经研究了免疫组织化学出生后（新生儿8周）的发展肾皮质基质细胞的5 'NT和细胞骨架蛋白α SMA和波形蛋白。α SMA和波形蛋白都是间质性肾炎中替代基质成纤维细胞的肾肌成纤维细胞的特征。在新生和1周龄大鼠中，皮质和髓质中的基质细胞显示α SMA和波形蛋白，但缺乏5 'NT。在出生后的第二周，α SMA和波形蛋白的免疫反应在皮质间质细胞逐渐下降，而5 'NT的反应变得越来越明显之间的曲小管在延髓迷路。在短时间内，发现所有三种蛋白质在相同的细胞中共表达。在第三周结束时，间质5 'NT-免疫反应性在表层皮质迷路中也变得明显，并且在皮质管周细胞中不再检测到α SMA和波形蛋白。从第4周开始，5 'NT阳性皮质成纤维细胞的分布模式和表型与成年大鼠一致。皮质管周成纤维细胞成熟的时间模式似乎与皮质小管的功能成熟平行。有人建议，在健康的地方表型的肾小管周围成纤维细胞，也可能在受伤的肾脏可以控制，至少部分地，由当地的肾小管环境，由肾小管代谢和功能的条件。
The stromal cells in the renal cortex and medulla of adult rats reveal different phenotypes. Cortical peritubular fibroblasts are ecto-5'nucleotidase (5'NT)-positive and lack alpha-smooth muscle actin (alpha SMA) and vimentin immunoreactivity, whereas medullary fibroblasts are 5'NT-negative and vimentin-positive. We have studied by immunohistochemistry the postnatal (neonatal up to 8 weeks) development of renal cortical stromal cells with respect to 5'NT and to the cytoskeletal proteins alpha SMA and vimentin. Both alpha SMA and vimentin are characteristic for the renal myofibroblasts that replace stromal fibroblasts in interstitial nephritis. In newborn and I-week-old rats, stromal cells in the cortex and medulla display alpha SMA and vimentin, but lack 5'NT. During the second postnatal week, alpha SMA and vimentin immunoreactivity in cortical interstitial cells gradually declines, whereas 5'NT reactivity becomes progressively apparent between the convoluted tubules in the juxtamedullary labyrinth. For a short time, all three proteins are found to be coexpressed in the same cells. At the end of the third week, interstitial 5'NT-immunoreactivity becomes evident also in the superficial cortical labyrinth, and alpha SMA and vimentin are no longer detectable in cortical peritubular cells. From the fourth week on, the distribution pattern and phenotype of 5'NT-positive cortical fibroblasts correspond to that in adult rats. The temporal pattern of maturation of cortical peritubular fibroblasts seems to parallel the functional maturation of cortical tubules. It is suggested that the local phenotype of peritubular fibroblasts in healthy and possibly also in injured kidneys may be controlled, at least in part, by the local tubular environment, conditioned by tubular metabolism and function.