Two type IV pili of Vibrio parahaemolyticus play different roles in biofilm formation
Two type IV pili of Vibrio parahaemolyticus play different roles in biofilm formation
复制标题
DOI：
10.1111/j.1574-6968.2006.00438.x
复制
发表时间：
2006-11-01
影响因子：
2.1
通讯作者：
Honda, Takeshi
中科院分区：
文献类型：
--
作者：
Shime-Hattori, Akiko;Iida, Tetsuya;Honda, Takeshi
副溶血性弧菌RIMD 2210633具有两组IV-A型菌毛基因。一组类似于在其他革兰氏阴性细菌中发现的，如铜绿假单胞菌、霍乱弧菌（几丁质调节菌毛; ChiRP）和创伤弧菌。另一个与甘露糖敏感血凝素（MSHA）菌毛的基因同源。在这项研究中，我们分析了每种IV型菌毛（ChiRP和MSHA菌毛）的菌毛蛋白基因缺失对生物膜形成的影响。虽然MSHA菌毛蛋白突变体形成聚集体，但直接附着于盖玻片的细菌数量减少，表明该菌毛有助于细菌附着于盖玻片表面。相比之下，ChiRP突变体附着于盖玻片表面，但不形成聚集体，表明ChiRP在生物膜形成期间在细菌凝集中起作用。这些结果表明，副溶血性弧菌的两种IV型皮利以不同的方式促进生物膜的形成。这两个突变体表现出较低的健身吸附到甲壳素颗粒比野生型。总的来说，这些数据表明，使用两种IV型皮利是副溶血性弧菌在自然环境中生存的一种改进策略。
Vibrio parahaemolyticus RIMD2210633 has two sets of type IV-A pilus genes. One set is similar to that found in other Gram-negative bacteria, such as Pseudomonas aeruginosa, Vibrio cholerae (chitin-regulated pilus; ChiRP), and Vibrio vulnificus. The other is homologous to the genes for the mannose-sensitive hemagglutinin (MSHA) pilus. In this study, we analyzed the effects of the deletions in the pilin genes for each type IV pilus (the ChiRP and the MSHA pilus) on biofilm formation. Although the MSHA pilin mutant formed aggregates, the number of bacteria that attached directly to the coverslip was reduced, suggesting that this pilus contributes to the bacterial attachment to the surface of the coverslip. In contrast, the ChiRP mutant attached to the surface of the coverslip, but did not form aggregates, suggesting that ChiRP plays a role in bacterial agglutination during biofilm formation. These results suggest that the two type IV pili of V. parahaemolyticus contribute to biofilm formation in different ways. Both mutants showed a lower fitness for adsorption onto chitin particles than that of the wild type. Collectively, these data suggest that the use of two type IV pili is a refined strategy of V. parahaemolyticus for survival in natural environments.