Molecular epidemiology of clinical isolates of methicillin-resistant Staphylococcus aureus in Taiwan
Molecular epidemiology of clinical isolates of methicillin-resistant Staphylococcus aureus in Taiwan
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DOI：
10.1128/jcm.42.1.307-310.2004
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发表时间：
2004-01-01
影响因子：
9.4
通讯作者：
Lin, TY
中科院分区：
文献类型：
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作者：
Huang, YC;Su, LH;Lin, TY
2000年7月至2001年10月期间，从分布于台湾北方、中部及南部的六个医疗中心，共收集到595株耐甲氧西林金黄色葡萄球菌（MRSA）临床分离株。标本来源包括血液（n = 279）、脓液（n = 173）、痰液（n = 94）、体液（n = 21）、导管头端（n = 20）和尿液（n = 8）。脉冲场凝胶电泳（PFGE）与SmaI消化用于指纹这些菌株。共鉴定出31种基因型和97种亚型。在7种基因型中可鉴定出亚型。虽然每家医院有6至15种基因型，但433株（73%）被证明属于一种主要类型（基因型A，有29种亚型）。该基因型不仅是所有6家医院的流行类型，而且也是每家医院的优势克隆，占每家医院所有分离株的46%至89%。基因型C（16种亚型）为第二优势基因型，占所有分离株的9%，分布于5家医院。基因型D（11种亚型）、E（5种亚型）和B（6种亚型）分别分布于5家、4家和3家医院。其余26种（30种亚型）为次要类型。我们的结论是，大多数MRSA临床分离株共享一个共同的PFGE模式，表明存在一个单一的，流行性MRSA克隆流行在台湾的主要医院。
During July 2000 and October 2001, a total of 595 clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) were collected from six medical centers distributed in northern, central, and southern Taiwan. Specimen sources included blood (n = 279), pus (n = 173), sputum (n = 94), body fluids (n = 21), catheter tips (n = 20), and urine (n = 8). Pulsed-field gel electrophoresis (PFGE) with SmaI digestion was used to fingerprint these isolates. A total of 31 genotypes with 97 type-subtypes were identified. Subtypes could be identified in 7 genotypes. While there were 6 to 15 genotypes in each hospital, 433 isolates (73%) were shown to belong to a major type (genotype A, with 29 subtypes). This genotype was not only the type prevailing in all six hospitals but also the predominant clone in each hospital, accounting for 46 to 89% of all isolates in each hospital. Genotype C (16 subtypes) was the second dominant genotype, accounting for 9% of all isolates, and was distributed in five hospitals. Genotypes D (11 subtypes), E (5 subtypes), and B (6 subtypes) were distributed in five, four, and three hospitals, respectively. The other 26 types (30 type-subtypes) were minor. We conclude that the majority of MRSA clinical isolates shared a common PFGE pattern, indicating the presence of a single, epidemic MRSA clone prevailing in major hospitals in Taiwan.