Comparative Transcriptome Analysis of Bombyx mori (Lepidoptera) Larval Hemolymph in Response to Autographa californica Nucleopolyhedrovirus in Differentially Resistant Strains
Comparative Transcriptome Analysis of Bombyx mori (Lepidoptera) Larval Hemolymph in Response to Autographa californica Nucleopolyhedrovirus in Differentially Resistant Strains
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家蚕（鳞翅目）幼虫血淋巴对差异抗性菌株中苜蓿银纹夜蛾核多角体病毒反应的比较转录组分析
DOI：
10.3390/pr9081401
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发表时间：
2021-08-01
期刊：
影响因子：
3.5
通讯作者：
Li, Mu-wang
中科院分区：
文献类型：
--
作者：
Ding, Xin-yi;Wang, Xue-yang;Li, Mu-wang
家蚕核多角体病毒（Bombyx mori nucleopolyhedrovirus，BmNPV）是一种给桑蚕生产造成巨大经济损失的病原菌。尽管苜蓿银纹夜蛾核多角体病毒（AcMNPV）和BmNPV都是杆状病毒，但这两种病毒的宿主结构域在本质上几乎没有交叉。近年来发现部分家蚕可通过穿刺方式感染重组AcMNPV，这为研究杆状病毒对家蚕的感染机制提供了有价值的材料。在本研究中，使用比较转录组学分析了接种 AcMNPV 后两种差异耐药菌株的血淋巴。病毒感染后，p50 中有 678 个 DEG，C108 中有 515 个 DEG。其中，p50中DEGs的上调和下调相似；然而，C108 中上调的 DEG 数量几乎是下调的 DEG 数量的两倍。不同抗性菌株中的DEG因GO富集而不同。基于KEGG富集，DEG主要富集于p50的代谢途径和C108的凋亡途径。此外，还分析了13个涉及代谢途径的基因和11个涉及细胞凋亡途径的基因。在参与细胞凋亡的DEG中，分析了BmTex261在病毒感染中的功能。 BmTex261在血淋巴中表现出最高表达，并且在C108的血淋巴中对病毒感染有显着反应，表明其参与抗AcMNPV感染。在 BmN 细胞中过表达 BmTex261 后，病毒基因 lef3 的表达显着降低，进一步验证了这一点。研究结果为家蚕抗BmNPV的分子机制提供了理论参考。
Bombyx mori nucleopolyhedrovirus (BmNPV) is a kind of pathogen that causes huge economic losses to silkworm production. Although Autographa californica nucleopolyhedrovirus (AcMNPV) and BmNPV are both baculoviruses, the host domains of these two viruses have almost no intersection in nature. Recently, it has been found that some silkworms could be infected by recombinant AcMNPV through a puncture, which provided valuable material for studying the infection mechanism of baculovirus to silkworm. In this study, comparative transcriptomics was used to analyse the hemolymph of two differentially resistant strains following AcMNPV inoculation. There were 678 DEGs in p50 and 515 DEGs in C108 following viral infection. Among them, the upregulation and downregulation of DEGs were similar in p50; however, the upregulated DEGs were nearly twice as numerous as the downregulated DEGs in C108. The DEGs in different resistant strains differed by GO enrichment. Based on KEGG enrichment, DEGs were mainly enriched in metabolic pathways in p50 and the apoptosis pathway in C108. Moreover, 13 genes involved in metabolic pathways and 11 genes involved in the apoptosis pathway were analysed. Among the DEGs involved in apoptosis, the function of BmTex261 in viral infection was analysed. The BmTex261 showed the highest expression in hemolymph and a significant response to viral infection in the hemolymph of C108, indicating that it is involved in anti-AcMNPV infection. This was further validated by the significantly decreased expression of viral gene lef3 after overexpression of BmTex261 in BmN cells. The results provide a theoretical reference for the molecular mechanism of resistance to BmNPV in silkworms.