Isolation and characterization of Drosophila retinal degeneration B suppressors.
Isolation and characterization of Drosophila retinal degeneration B suppressors.
复制标题
果蝇视网膜变性 B 抑制因子的分离和表征。
DOI：
10.1093/genetics/151.2.713
复制
发表时间：
1999
期刊：
影响因子：
3.3
通讯作者：
Hyde,DR
中科院分区：
文献类型：
--
作者：
Paetkau,DW;Elagin,VA;Sendi,LM;Hyde,DR
The Drosophila retinal degeneration B protein (RdgB) is a novel integral membrane phosphatidylinositol transfer protein required for photoreceptor cell viability and light response. We isolated one intragenic suppressor (rdgBsu100) and four autosomal suppressors of the hypomorphic rdgBKS222retinal degeneration phenotype. The rdgBsu100suppressor dramatically slowed rdgBKS222's photoreceptor degeneration without significantly improving the electroretinogram (ERG) light response. One autosomal recessive suppressor [su(rdgB)69] significantly slowed rdgBKS222retinal degeneration and restored the ERG light response near to that of the wild type. Unlike all the previously characterized rdgB suppressors, the four new autosomal suppressors do not affect the ERG light response in rdgB+flies. Only Su(rdgB)116 exhibited a mutant phenotype in a rdgB+background, which was smaller R1-6 rhabdomeres. We also examined the extent to which two previously identified visual transduction mutations suppressed rdgB retinal degeneration. Absence of one of the light-activated calcium channels (trpCM) slowed the onset of rdgB-dependent degeneration. However, loss of protein kinase C (inaC209), which blocks photoreceptor cell deactivation, desensitization, and light adaptation, failed to suppress rdgB degeneration under normal light conditions. This demonstrates that TRP activity, but not INAC, is required for rapid rdgB-dependent degeneration.
