Resistance of red clover (Trifolium pratense) to the root parasitic plant Orobanche minor is activated by salicylate but not by jasmonate
Resistance of red clover (Trifolium pratense) to the root parasitic plant Orobanche minor is activated by salicylate but not by jasmonate
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DOI：
10.1093/aob/mcm148
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发表时间：
2007-09-01
期刊：
影响因子：
4.2
通讯作者：
Yoneyama, Koichi
中科院分区：
文献类型：
--
作者：
Kusumoto, Dai;Goldwasser, Yaakov;Yoneyama, Koichi
背景与目的专性根全息寄生虫侵染双子叶作物，在世界许多地区造成严重损失。化学诱导植物防御系统如系统获得性抗性被认为是一种有效的控制根寄生虫的策略，但所涉及的详细机制尚未明确。研究了水杨酸（SA）、茉莉酸（JA）及其类似物对红三叶草寄生抗性的影响。方法用SA、s -甲基苯并[1,2,3]噻二唑-7-碳硫酸盐（BTH）、茉莉酸甲酯（MeJA）和正丙基二氢茉莉酸甲酯（PDJ）对红三叶草根进行接种，然后接种O. minor种子。5周后观察寄生虫的附着。SA和BTH是SA介导防御的诱导剂，可显著减少已建立寄生虫的数量，减少幅度超过75%。相比之下，ja介导的防御诱导剂MeJA和PDJ对寄生没有影响。SA和BTH减少寄生物数量是由于抑制了O. minor根的伸长和激活寄主根的防御反应，包括内胚层的木质化。结论sa诱导的抗虫能力明显优于ja诱导的抗虫能力，且抗虫能力是通过寄主根系内外表达的。
Background and Aims Obligate root holoparasites of the genus Orobanche attack dicotyledonous crops and cause severe losses in many parts of the world. Chemical induction of plant defence systems such as systemic acquired resistance was proposed to be an available strategy to control the root parasite, but the detailed mechanisms involved have not been clarified. The aim of this study was to elucidate the effects of salicylic acid (SA), jasmonic acid (JA) and their analogues on resistance of red clover to Orobanche parasitism.Methods Roots of red clover grown in plastic chambers were applied with SA, S-methyl benzo[1,2,3]thiadiazole-7-carbothioate (BTH), methyl jasmonate (MeJA) and n-propyl dihydrojasmonate (PDJ), and then were inoculated with O. minor seeds. Attachments of the parasite were observed after 5 weeks.Key Results SA and BTH, inducers of SA-mediated defences, significantly reduced the number of established parasites by more than 75%. By contrast, MeJA and PDJ, inducers of JA-mediated defences, did not affect parasitism. The reduction in the number of established parasites by SA and BTH was due to the inhibited elongation of O. minor radicles and the activation of defence responses in the host root including lignification of the endodermis.Conclusions These results suggest that SA-induced resistance, but not JA-induced resistance, is effective in inhibiting Orobanche parasitism and that the resistance is expressed by the host root both externally and internally.