Use of Folded Micromachined Pillar Array Column with Low-Dispersion Turns for Pressure-Driven Liquid Chromatography
Use of Folded Micromachined Pillar Array Column with Low-Dispersion Turns for Pressure-Driven Liquid Chromatography
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DOI：
10.1021/ac902491x
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发表时间：
2010-02-15
影响因子：
7.4
通讯作者：
Tsunoda, Makoto
中科院分区：
文献类型：
--
作者：
Aoyama, Chiaki;Saeki, Akira;Tsunoda, Makoto
在这项研究中，我们首次表明，使用具有低分散匝数的分离通道可以提高压力驱动液相色谱（LC）条件下柱阵列柱的分离效率。柱阵列柱是通过硅基板的反应离子蚀刻制造的。利用低色散匝几何结构，可以在 20 x 20 mm 微芯片上制造长度和宽度分别为 110 mm 和 400 μm 的柱。在非保留条件下，荧光化合物获得的溶质带即使在通过低色散圈后也几乎保持不变；然而，在恒定半径转弯的情况下观察到溶质带的显着偏斜。两种香豆素染料在反相条件下得到良好的分离，最大理论塔板数为8000。 140秒内成功分离了6种氨基酸的荧光衍生物。这些结果表明，使用低分散圈数的长分离通道可以显着提高微芯片色谱的分离效率。
In this study, we show for the first time that the separation efficiency of a pillar array column under pressure-driven liquid chromatography (LC) conditions can be improved using a separation channel with low-dispersion turns. The pillar array column was fabricated by reactive ion etching of a silicon substrate. With the low-dispersion-turn geometry, a column with a length and width of 110 mm and 400 mu m, respectively, could be fabricated on a 20 x 20 mm microchip. Under nonretained conditions, the solute bands obtained for fluorescent compounds remained almost unchanged even after passing through the low-dispersion turns; however, significant skewing of the solute bands was observed in the case of constant-radius turns. Two coumarin dyes were well resolved under reversed-phase conditions, and a maximum theoretical plate number of 8000 was obtained. Successful separation of the fluorescent derivatives of six amino acids was achieved in 140 s. These results indicated that the separation efficiency of microchip chromatography could be significantly improved using a long separation channel with low-dispersion turns.