DIFFERENTIAL-EFFECTS OF ENDOTHELIN-1 AND BIG ENDOTHELIN ON CANINE KIDNEYS
DIFFERENTIAL-EFFECTS OF ENDOTHELIN-1 AND BIG ENDOTHELIN ON CANINE KIDNEYS
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DOI：
10.1097/00005344-199100177-00086
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发表时间：
1991-01-01
影响因子：
3
通讯作者：
OMAE, T
中科院分区：
文献类型：
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作者：
AKABANE, S;YOSHIMI, H;OMAE, T
检测了内皮素-1（ET-1，人，1-21）和大ET（人，1-38）的等效摩尔浓度的肾脏效应以及洋地黄素和内皮源性舒张因子对这些作用的贡献。 以0.4 pmol/kg/min的剂量肾内输注ET-1，使肾血流量（RBF）、肾小球滤过率（GFR）和尿量（V）分别降低至对照值的62%、70%和45%。 额外的阿司匹林-DL-赖氨酸（ASP）处理（25 mg/kg）导致RBF、GFR和V分别显著降低至对照值的20%、14%和9%。 在ET-1输注期间，肾内输注N（G）-单甲基-L-精氨酸（L-NMMA，1 μ mol/min）引起RBF、GFR和V降低。在另一组中，肾内输注相当于ET-1剂量的大ET对RBF、GFR和尿量无显著影响，分别为对照值的95%、107%和84%。 额外的ASP处理将这些值分别降低至对照值的78%、69%和52%。 大剂量ET输注期间输注L-NMMA对肾功能无明显影响。 我们的研究表明，肾内注射ET-1，在0.4 pmol/kg/min的剂量，引起显着的血流动力学和功能的变化，在犬肾脏相比，等摩尔浓度的大ET。
Renal effects of endothelin-1 (ET-1, human, 1-21) and the equivalent molar concentration of big ET (human, 1-38) and the contributions of prostaglandins and endothelium-derived relaxing factor to these actions were examined. Intrarenal infusion of ET-1, at a dose of 0.4 pmol/kg/min, decreased renal blood flow (RBF), glomerular filtration rate (GFR), and urine output (V) to 62, 70, and 45% of control values, respectively. Additional aspirin-DL-lysine (ASP) treatment (25 mg/kg) caused marked decreases in RBF, GFR, and V to 20, 14, and 9% of control values, respectively. During ET-1 infusion, intrarenal infusion of N(G)-monomethyl-L-arginine (L-NMMA, 1-mu-mol/min) caused decreases in RBF, GFR, and V. In another group, intrarenal infusion of big ET at a dose equivalent to that of ET-1 did not significantly affect RBF, GFR, or urine output, which were 95, 107, and 84% of control values, respectively. Additional ASP treatment decreased these values to 78, 69, and 52% of control values, respectively. L-NMMA infusion during big ET infusion showed little effect on renal function. Our study demonstrated that intrarenal infusion of ET-1, at a dose of 0.4 pmol/kg/min, caused significant hemodynamic and functional changes in the canine kidney compared with an equivalent molar concentration of big ET.